Daporinad (FK866) is among the highly specific inhibitors of nicotinamide phosphoribosyl transferase (NAMPT) and recognized to have its mechanism of action that induces the tumor cell apoptosis. Within this study, an easy and sensitive liquid chromatography-quadrupole-time-of-flight-mass spectrometric (LC-qTOF-MS) assay continues to be produced for the look at drug metabolic process and pharmacokinetics (DMPK) qualities of Daporinad in rodents. An easy protein precipitation method using acetonitrile (ACN) was utilized for that sample preparation and also the pre-treated samples were separated with a C18 column. The calibration curve was evaluated in the plethora of 1.02~2220 ng/mL and also the quadratic regression (weighted 1/concentration2) was utilized to find the best fit from the curve having a correlation coefficient ≥ .99. The qualification run met the acceptance criteria of ±25% precision and precision values for QC samples. The dilution integrity was verified for five, 10 and 30-fold dilution and also the precision and precision from the dilution QC samples were also acceptable within ±25% from the nominal values. The soundness results established that Daporinad was stable for an additional conditions: short-term (4 h), lengthy-term (2 days), freeze/thaw (three cycles). This qualified method was effectively put on intravenous (IV) pharmacokinetic (PK) studies of Daporinad in rodents at doses of 5, 10 and 30 mg/kg. Consequently, it demonstrated a straight line PK inclination within the dose vary from five to ten mg/kg, however a non-straight line PK inclination within the dose of 30 mg/kg. Additionally, in vitro as well as in vivo metabolite identification (Met ID) studies were conducted to know the PK qualities of Daporinad and also the results demonstrated that as many as 25 metabolites were recognized as ten various kinds of metabolic process within our experimental conditions. To conclude, the LC-qTOF-MS assay was effectively produced for the quantification of Daporinad in mouse plasma and for its in vitro as well as in vivo metabolite identification.Darapladib