In this work, we established the first CRISPRa system for filamentous fungi. Initially, we built a CRISPR/dLbCas12a-VPR-based system and demonstrated the activation of a fluorescent reporter in Aspergillus nidulans. Then, we targeted the local nonribosomal peptide synthetase-like (NRPS-like) gene micA in both chromosomal and episomal contexts, achieving increased production of the ingredient microperfuranone. Finally, multigene CRISPRa resulted in the development associated with the mic group product as dehydromicroperfuranone. Also, we demonstrated the energy associated with variant dLbCas12aD156R-VPR for CRISPRa at room temperature culture conditions. Different aspects that manipulate the effectiveness of CRISPRa in fungi had been investigated, offering a framework for the additional growth of fungal artificial transcription aspects predicated on CRISPR/Cas.The article is dedicated to M. N. Akhutin (1899-1848), one of several founders regarding the Russian army field surgery, lieutenant-general of the medical service, professor, corresponding person in the USSR Academy of medical Sciences, chief of the Military medical SARS-CoV-2 infection Academy (1940-1941). M. N. Akhutin, disciple of V. A. Oppel, had been energetic participant in the battles during the lake Hasan while the Khalkhin-Gol lake (1838-1939). Through the Great Patriotic War, he had been the main physician regarding the Bryansk, 2nd Baltic and 1st Ukrainian fronts. Since 1945 he ended up being deputy chief surgeon of the Soviet Army. He could be author of works on army area surgery. He had been the very first director associated with A. V. Vishnevsky institute of surgery and the head of this division of faculty surgery associated with the I. M. Sechenov 1st Moscow medical Institute.The article reveals the problems for creation of idea of “the Soviet medicine” and traces the advancement of their concepts in other words. the understanding of its crucial features. The foundation base was made on removed their particular formulations from the program texts of Bolsheviks, journal journals of health directors, mins of meetings of historians of medicine and textbooks in the company of health care. The research demonstrated just how through the 100 years (1918-2018) the semantics of formulations changed quite drastically. The reason is suggested concerning exactly how and exactly why the previous meanings were lost plus the form of statements sacralized.The article presents the results of analysis associated with quantity and construction of physicians and professionals with additional health education into the Moscow healthcare system. The outcomes of study demonstrated that in 2019, the help associated with the populace with physicians and professionals with secondary medical education diminished as compared with 2014 from 39.4 to 37.1 and from 74.2 to 55.7 (per 10 thousand people) respectively. How many busy health opportunities increased from 84.6% in 2014 to 87percent in 2019, the staffing prices (for busy positions of secondary medical workers) also increased from 84.9% to 87.4 per cent. It was set up that the staffing of an individual, both for physicians and paramedical personnel, increased from 66.8% to 79.8per cent and from 69.4% to 79.0percent, respectively. The concurrency coefficient had been 1.1 (for medical jobs and roles of additional health personnel). The problems associated with reasonable staffing levels and large co-employment prices for individual health jobs and roles of secondary health personnel require further resolution.The article views the relevance of marketing analysis in the area of paid medical services. Because of the fact that industry of paid solutions in Russia has grown dramatically, medical administration took neighboring place to medical advertising. The content emphasizes that in Russia annually the sheer number of exclusive health establishments increases. This trend is right associated with to your undeniable fact that no-cost health care is not constantly in a position to supply essential services important for the client. In change, the existing increased competition pushes personal medical establishments to definitely fight for the customer. The statistical data demonstrates that the loss of five customers per day leads to losing as much as two million rubles month-to-month. It id founded that because of more and more exclusive health institutions an urgent dependence on brand new logistics and marketing analysis of all of the medical manufacturing occurs having more extensive picture of the provision of a paid health care. It is concluded that achieving these advertising and marketing and marketing study jobs determine this new special host to advertising technologies. The advertising and marketing in the health care system acts as fundamental system of principles geared to producing and studying the need and provide of health services. In the healthcare marketplace, the part for the customer is played by patients whose needs should be satisfied by medical clinics.