, 2010; Marciano-Cabral et al., 2010), bacterial intracellular position can consequently protect it from adverse conditions. HTS assay Moreover, similar studies may be conducted with strains resistant to antibiotics in order to evaluate, as regards Mycobacterium smegmatis, the potential intracellular persistence of such strains (Sharbati-Tehrani et

al., 2005). The ability of A. baumanii to grow and survive intracellularly in Acanthamoeba species may be one factor that could enhance bacterial survival in aquatic environments and networks. Hence, in hospital water taps, special attention should be paid to the presence of free-living amoebae, which can promote survival of this pathogenic bacteria. “
“A Gram-negative, facultatively anaerobic, motile and slightly curved rod-shaped bacterium

(BFLP-4T) was isolated from the faeces of wild seahorses (Hippocampus guttulatus) captured in northwest Spain (Toralla, Galicia). Strain BFLP-4T grew at 10–35 °C and pH 5–9 (optimally at 20 °C and pH 7.2) and at salt concentrations in the range 0–7% w/v NaCl. The G+C content of the DNA was 49.3 mol%. Phylogenetic analysis based on the 16S rRNA gene sequence showed that strain BFLP-4T was a member of the genus Vibrio, being most closely related to Vibrio ichthyoenteri (97.1%), Vibrio mediterranei (96.7%), Vibrio scophthalmi (96.7%) and Vibrio sinaloensis (96.6%). A phylogenetic analysis based on recA Pritelivir clinical trial gene sequences also supported the affiliation of strain BFLP-4T to the genus Vibrio. Strain BFLP-4T could be readily differentiated from other closely related species by several phenotypic properties and fatty acid profiles. On the basis of phenotypic, chemotaxonomic and phylogenetic data, strain BFLP-4T represents a novel species within Methane monooxygenase the genus Vibrio, for which the name Vibrio hippocampi sp. nov. is proposed. The type strain is BFLP-4T (=DSM 22717T=LMG 25354T). The genus Vibrio comprises a genetically diverse group of heterotrophic marine bacteria that are found in a variety of aquatic environments (Thompson

et al., 2004). Vibrio species are commonly found as members of the normal microbiota in marine invertebrates and fish, but they are also found to be aetiological agents of several diseases in humans and aquatic animals (Tantillo et al., 2004; Thompson et al., 2004; Igbinosa & Okoh, 2008; Balcázar et al., 2010). In the present study, we describe the physiological, chemotaxonomic and phylogenetic characteristics of a Gram-negative, motile, facultatively anaerobic and slightly curved rod-shaped bacterium sharing the highest 16S rRNA gene sequence similarities to Vibrio ichthyoenteri DSM 14397T, Vibrio mediterranei CIP 103203T, Vibrio scophthalmi A089T and Vibrio sinaloensis CAIM 797T. During the characterization of organisms isolated from faeces of wild seahorses (Hippocampus guttulatus), strain BFLP-4T was grown on tryptone soy agar (TSA) supplemented with 1.5% NaCl (w/v) at 20 °C for 72 h.