In the first variant, animals get into a dark compartment from an open illuminated platform (platform), whereas in the other, from an enclosed illuminated one (box). PIC impaired retention performance in the “”platform-type”" IA, but not in the “”box-type”". DZP enhanced retention performance in both types of IA task. These results evidence critical differences between the two step-through inhibitory avoidance tasks used, that might be relevant not only for retention performance Selleckchem PD0325901 during memory retrieval, but also for the theoretical interpretations and conclusions obtained from behavioral results.
(C) 2008 Elsevier Ireland Ltd. All rights reserved.”
“Heme oxygenase (HO), the main enzyme deputed to heme metabolism, has been identified as two main isoforms called HO-1 and HO-2. HO-1 is inducible and plays a main role in the cellular oxidant/antioxidant balance whereas HO-2 is constitutive and involved in the physiological metabolism of heme. However, it is noteworthy
to 8-Bromo-cAMP ic50 mention that HO contribute to the regulation of the hypothalamic release of neuropeptides such as corticotrophin-releasing hormone and arginine-vasopressin and could modulate the pulsatile release of gonadotropin releasing hormone (GnRH). GT1-7 cells are immortalized hypothalamic neurons and a valuable tool to evaluate hypothalamic neuroendocrine control of reproduction. The aim of this work was to investigate and characterize the presence of HO isoforms in the GT1-7 hypothalamic neurons. Hemin, a well-known inducer of HO-1, significantly increased HO activity, whereas dexamethasone did not modify HO-2 activity. Moreover, hemin and DEX, in combination, did not have any additive effect on HO activity in GT1-7 neurons. Furthermore, basal HO-1 immunoreactivity,
identified in GT1-7 cells, was significantly up-regulated by hemin. Conversely, no HO-2 immunoreactivity was detected. Taken together, these results suggest the presence of functional HO-1 in GT1-7 immortalized hypothalamic neurons and open new avenues about the use of through this cell line for the study of HO modulation of GnRH secretion and reproduction. (C) 2008 Elsevier Ireland Ltd. All rights reserved.”
“Purpose: We identified significantly hypermethylated genes in clear cell renal cell carcinoma.
Materials and Methods: We previously identified a set of under expressed genes in renal cell carcinoma tissue through transcriptional profiling and a robust computational screen. We selected 19 of these genes for hypermethylation analysis using a rigorous search for the best candidate regions, considering CpG islands and transcription factor binding sites. The genes were analyzed for hypermethylation in the DNA of 38 matched clear cell renal cell carcinoma and normal samples using matrix assisted laser desorption ionization time-of-flight mass spectrometry. The significance of hypermethylation was assessed using 3 statistical tests.