, 2002).

The same blood flow apparatus previously described in this journal was used ( Chen et al., 2012b) (with updated oxygenators), but this time employing a computer controlled system to activate the switching of blood flows at varying duty cycles and simulated respiratory rates (RR). Galunisertib datasheet Cyclic variations in the oxygenation of blood within the respiratory cycle were initially reported in 1961 (Bergman, 1961a and Bergman, 1961b). Several studies, presented and discussed in more detail in the discussion section, have explored the nature of these oscillations, especially in association with cyclical atelectasis in the lung, observed in the Acute Respiratory

Distress Syndrome. Overall, these studies clearly indicate that very fast PaO2PaO2 and SaO2 sensors are needed to follow, in real time, dynamic changes in arterial blood oxygen tension – and that a fast response blood-flow test apparatus is needed to ascertain if this new generation of optical oxygen sensors is fit for purpose. With this background in mind, we PF-01367338 ic50 decided to modify the existing cross-over liquid flow apparatus (Chen et al., 2012b) to simulate cyclical pulmonary shunt changes with different I  :E   ratios and RRs. This Thymidylate synthase would enable the in-house sensor, as well as the commercial Foxy AL 300 sensor, to be tested to examine if they had a fast enough time response to measure faithfully very fast oscillations in PaO2PaO2 on-line in flowing blood, and to investigate if a diminution in ΔPaO2 with increasing RR could be due to sensor technology limitation or might be a true physiological phenomenon ( Baumgardner et

al., 2002). We also tested whether or not our in-house sensor was resistant to clot formation when exposed to flowing blood for a 24-h period in vivo. We investigated the capacity of an in-house, custom-built fibre optic PO2PO2 sensor to detect rapid PO2PO2 oscillations in blood in vitro  . This sensor is made by coating the end section of a silica fibre with a Pt(II) doped polymer sensing material, poly(methyl methacrylate) (PMMA). This PMMA sensor is based on the principle of fluorescence quenching of the platinum complex by oxygen, and is compatible with clinical application. Further technical details about the sensor have been reported previously ( Chen et al., 2012a). The Foxy-AL300 fibre optic PO2PO2 sensor was used as a control for comparison with the PMMA sensor. Each sensor was calibrated in blood at 0 and 50 kPa before each experiment.