2b). The transiently transfected cells indeed responded to treatment with 15 μM PEITC involving a disintegration of the microtubular filaments over a 25–35-min period followed by the formation Ixazomib ic50 of apoptotic blebs surrounding the cells (Fig. 2b,c). Cells treated with vehicle control were observed for up to 1 h without any apparent changes in the microtubular network or formation of apoptotic blebs (Fig. 2d), which suggests that treatment of Kato-III cells with PEITC leads to deformation of microtubular filaments to presumably contribute to a shift in the cell cycle distribution and ultimately induction of apoptosis. As MKN74 cells responded differently to PEITC treatments compared

with Kato-III cells

when assayed for the effects on proliferation, cell cycle distribution, and appeared to form less apoptotic blebs, these cells were further investigated in order to elucidate if these cells in fact became significantly apoptotic by PEITC treatments. Subjecting MKN74 cells treated with PEITC for 48 h to a flow cytometry-based apoptosis assay kit resulted in an increase in the relative number of apoptotic cells from 10% in the vehicle control treated culture to 13% and 16% in the cultures treated with 5 and 15 μM, respectively (Fig. 3a). Y-27632 in vitro The weak response in apoptotic cells coincided with the findings when PEITC-treated MKN74 cells were analyzed for caspase-3 activity (Fig. 3b). Cells were treated with 5–15 μM PEITC for 48 h before harvested and analyzed for caspase-3 activity which revealed a weak dose-dependent effect of PEITC on caspase-3 activity in MKN74 cells. Further, we investigated the effect of PEITC on the GSH pool of MKN74 cells as ITCs readily bind to the sulfhydryl group of GSH which may disturb the intracellular redox balance in cells. The reduction selleck chemical of total intracellular GSH in MKN74 cells treated with 1–5 μM PEITC for 5 h was approximately 20% and, surprisingly, did not respond dose-dependently (Fig. 3c). This reduction was presumably insufficient

to disturb the intracellular redox homeostasis and further induce secondary effects such as increased reactive oxygen species (ROS) level. Flow cytometric analysis of MKN74 cells treated with 1–5 μM PEITC for 5 h showed no significant differences in ROS levels (data not shown). The dietary plant phytochemical PEITC is thought to contribute to chemoprevention against several types of human cancer diseases alongside with other plant phytochemicals enriched in a diet with cruciferous vegetables. Moreover, PEITC has also been suggested for clinical treatment of cancer as it may cure resistance to cancer drugs and thus sensitize cancer cells to these drugs.[22] It is therefore important to understand the underlying mechanisms upon the PEITC entry to a cancer cell.