3(D)). Specifically, the developmental change in mineral particle angle with development is similar for both LB and IF. Starting from a lower degree of misalignment (~ 60°) at 1 week (compared to ~ 110–130° for wild type mice), the decrease of angle in both anatomical regions is similar (~ 85%). A subsequent slight increase is not statistically significant (p > 0.05). Fig. 4 shows the values of ρ as a function of anatomical region Selleck LY294002 and disease condition for all developmental ages. In the wild-type animals ( Fig. 4A dotted line), the degree of orientation in the LB (bony ridge) increased significantly with age (p < 0.01). The most significant increment in the

degree of orientation (p < 0.01) was observed between 1 week and 4 weeks in wild-type mice scapulae ( Fig. 4B). After 4 weeks of age, the degree of orientation does not increase to the same extent. In contrast, at the IF, no statistically JQ1 chemical structure significant difference in degree of orientation with age was observed (p > 0.05). In Hpr mice ( Fig. 4A dash line), in contrast, the degree of mineral crystallite orientation in both regions increases significantly (IF and LB: p < 0.01) ( Fig. 4C).

Intra-sample t-tests show that the significant increase is from 1 to 7 weeks for both regions (p < 0.01). Therefore, the difference between the LB and the IF is lost. These results showed that, in wild-type mice scapulae, the degree of orientation of the mineral crystals is greater at sites where higher muscle forces are exerted. Methamphetamine From the histograms of degree of mineralisation (measured using micro-CT), the mean mineral concentration was plotted as a function of

age for LB (Fig. 5(A)) and IF (Fig. 5(B)), for both wild type and Hpr mice. The mean mineral concentration in wild type and Hpr mice was similar at 1 week for both the LB and the IF (Fig. 5(A)). The rate of increase in mineralisation with age was greater in wild type mice compared to Hpr mice (Fig. 5(B)) in the LB. However, in the flat infraspinous region, the rates of increase were similar for wild type and Hpr mice. The mean mineral concentration was lower at the IF compared to the LB in wild type mice at every age, and the difference became more significant (p < 0.05) with age. These variations across the scapula in wild-type mice show that increase in mineral content with age was greater at sites where higher muscle forces are exerted. From the foregoing, it is evident that our results demonstrate an association between muscular forces acting on the bone, and bone-matrix nanostructure with development in intramembranously ossified bones, and that a significant disruption of this correlation occurs under the conditions of hypomineralisation [21] and reduced muscular forces [22] observed in murine models of rickets. With scanning synchrotron SAXS [18], we were able to map microscale variations in bone nanostructure at different stages of tissue maturity.