95 0 43             x    

  tblastx EU399681 1 Glutathion

95 0.43             x    

  tblastx EU399681.1 Glutathione peroxidase Metapenaeus ensis 5E-36 0.71 0.57                   Cu/Zn SOD blastx ABU55006.1 Copper/zinc superoxide dismutase Macrobrachium rosenbergii 1E-30 0.43 0.47 x           x       tblastx EU077527.1 Copper/zinc superoxide dismutase Macrobrachium rosenbergii 9E-32 0.31 0.71 click here                   cytMnSOD blastx CAR85669.1 cytoplasmic manganese superoxide dismutase Cyanagraea praedator 2E-102 0.68 0.66 x       x   x       tblastx FM242568.1 cytoplasmic manganese superoxide dismutase Cyanagraea praedator 8E-116 0.68 0.73                 Coagulation Transglutaminase B blastx AAK69205.1 Transglutaminase Pacifastacus leniusculus 3E-70 0.78 0.54 x           x       tblastx AF336805.1 Transglutaminase Pacifastacus leniusculus 8E-84 0.78 0.60                 Cellular differentiation Astakine blastx ACI02322.1 astakine variant 2 Penaeus monodon 3E-11 0.64 0.52             x       tblastx EU980445.1 Trichostatin A molecular weight astakine variant 2 Penaeus monodon 7E-15 0.72 0.49                   Runt blastx CAD44571.1 runt protein 1b Pacifastacus leniusculus 2E-45 0.67 0.65           x         tblastx AJ506096.1 Pacifastacus

leniusculus mRNA for runt protein Pacifastacus leniusculus 8E-73 0.65 0.82                 Apoptosis AIF-like blastx NP_001121885.1 apoptosis-inducing factor Danio rerio 7E-28 0.54 0.43             x       tblastx NM_001128413.1 apoptosis-inducing factor Danio rerio 9E-30 0.52 0.49                 Cyclin-dependent kinase 3 Autophagy ATG7 blastx XP_002600056.1 hypothetical protein BRAFLDRAFT_79689 Branchiostoma floridae 2E-40 0.88 0.52       x             tblastx NM_001129922.1 ATG7 autophagy related 7 homolog Xenopus tropicalis 5E-40 0.68 0.61                   ATG12

blastx ADO32996.1 Autophagy-like protein ATG12 Biston betularia 3E-33 0.50 0.52       x             tblastx HM449861.1 Autophagy-like protein ATG12 Biston betularia 1E-38 0.47 0.53               Other Cytoskeleton Kinesin blastx NP_999817.1 kinesin II Strongylocentrotus Selleck LCZ696 purpuratus 3E-159 0.81 0.83         x   x       tblastx NM_214652.1 kinesin II Strongylocentrotus purpuratus 0.0 0.82 84.00               Immune gene expression The expression of 46 candidate immune genes (Table 4 and Additional File 1: Primer pairs used for RT-qPCR quantification) were quantified in whole animal, ovaries and immune tissues of symbiotic and asymbiotic A. vulgare females. Forty four genes were selected through the procedure described above and 2 other genes were selected from previous studies [44, 45]. Twelve genes were selected from the SSH-C (11 unigenes) and SSH-NC (1 unigene) libraries in order to examine whether Wolbachia induce an immune activation as observed in a challenged condition. All the 46 selected immune genes can be placed in known crustacean immune pathways (Figure 3).

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