c Section through peridium. d Pseudoparaphyses. e−f Asci. g Asci with pseudoparaphyses. h−k Ascospores. Scale bars:

a = 500 μm, b = 200 μm, c−d, g = 50 μm, e−f = 20 μm, h−k = 10 μm ≡ Botryosphaeria subglobosa (C. Booth) Arx & E. Müll., Stud. Mycol. 9: 15 (1975) ≡ Coniothyrium subglobosum (Cooke) Tassi, Bulletin Labor. Orto Bot. de R. Univ. Siena 5: 25 (1902) = Macroplodia subglobosa (Cooke) Kuntze, Revis. gen. pl. 3: 492 (1898) ≡ Sphaeropsis subglobosa Cooke, Grevillea 7(no. 43): 95 (1879) Saprobic on dead bamboo. Ascostromata 140–200 μm high, 210–360 μm diam, dark brown, uniloculate, semi-immersed in host tissue, with protruding papilla or erumpent, developing under raised, dome-shaped regions. Ostiole 45–75 × 50–80 μm, central, papillate. Peridium 15–40 μm wide, comprising several layers of dark brown-walled cells of textura angularis. Pseudoparaphyses up FDA-approved Drug Library in vivo to 3–5 μm wide, hyphae-like, cellular, BMS345541 numerous, embedded in a hyaline gelatinous matrix. Asci (70-)81.5–100(−117) × 18–22.5(−23) μm \( \left( \overline x = 89.2 \times 20.7\,\upmu \mathrmm,\mathrmn = 20 \right) \), 8-spored, bitunicate, fissitunicate,

clavate to cylindro-clavate, with a short rounded pedicle, apically rounded with an ocular chamber (2.5–4.5 μm wide, n = 5). Ascospores (19.5-)21–26(−28) × (6.5-)7.5–9.5(−10) μm \( \left( \overline x = 23.4 \times 8.5\,\upmu \mathrmm,\mathrmn = 30 \right) \), uniseriate at the base, biseriate at the apex, hyaline, aseptate, ellipsoidal to fusiform, usually widest in the middle, rough-walled, with bipolar germ pores, surrounded by distinctive structured mucilaginous sheath. Pycnidia 150–200 μm diam., brown to black, solitary or aggregated sometimes intermixed amongst ascostromata, unilocular or multilocular,

spherical to globose, wall stromatic, composed of several layers of laterally compressed brown cells. Conidia (phialospores) 9–12 × 6–9 μm, mature ones light brown to dark brown, spherical to subglobose (asexual morph description follows Punithalingam 1969). Material examined: SIERRA LEONE, Njala (Kori), on dead culms of Bambusa arundinacea, 17 August 1954, F.C. Deighton (IMI 57769 c, holotype); THAILAND, Lampang Province., Jae Hom District, Mae Yuag Forestry Plantation, on dead culms of Bambusa sp., 19 August 2010, Erythromycin R. Phookamsak, RP0079 (MFLU 11–0199), living culture MFLUCC 11–0163. Notes: MFLU 11–0199 is a fresh collection of Neodeightonia subglobosa from Bambusa sp., and is similar to N. palmicola, which also has hyaline, aseptate ascospores surrounded by a www.selleckchem.com/products/ganetespib-sta-9090.html wing-like hyaline sheath. However, MFLU 11–0199 differs from N. palmicola in having smaller asci and ascospores lacking bipolar germ pores. The original description of N. subglobosa reported that the ascospores become 1–septate, and brown to dark brown when mature, and this was not observed in N. palmicola and no asexual morph was formed in culture. In Fig. 1 the new isolate clustered together with a strain of N. subglobosa (CBS 448.