Daily treatments of TSA reduced severity of experimental autoimmune encephalomyelitis (EAE) as determined by the disease index score and down-regulation of Th1-related cytokines. This study did not examine a role for Treg cell enhancement as a result of TSA treatments. However, other studies have directly assessed for TSA-mediated enhancement on the generation and activity of Treg cells [12]. Daily TSA injections for 7 days were shown to boost the percentage of murine Treg

cells in vivo. The authors used three models to investigate whether this increase was owing to conversion of naïve CD4+FoxP3− T cells into CD4+FoxP3+ Treg cells. Treg cell conversion was only seen when natural Treg cells were first depleted from the mouse. This finding led the investigators to conclude that a beneficial

in vivo effect was due to an increase in thymic output of natural Treg cells in both https://www.selleckchem.com/products/SRT1720.html the spleen and lymph nodes. Furthermore, Treg cells isolated from TSA-treated mice were more suppressive on a per cell basis than Treg cells from control MLN2238 mice. Yet despite these findings, other investigators concluded that daily treatments of TSA may impair splenic CD4+FoxP3+ Treg cell numbers in vivo [25]. Additionally, FoxP3 mRNA procured from splenocytes was decreased in TSA-treated mice. In vitro assays detailed that FoxP3 mRNA appeared unstable after administration of TSA. It is unclear if TSA treatments yield various competing direct and/or indirect effects that may explain the different results noted by these authors. The differences did not appear to depend on in vitro or in vivo testing, as another study performed by Moon et al. [26] revealed that TSA induced FoxP3 protein within mitogen-stimulated CD4+FoxP3− T cells. A timed treatment of TSA 72 h into the culture induced FoxP3 protein for the following 72 h. FoxP3 protein was no longer detectable after that period, which indicated that singular treatments of HDAC inhibitors may only temporarily induce Treg cells. The current study showed

that the short-chain fatty acid n-butyrate could Grape seed extract induce functional unresponsiveness in CD4+ T cells independent of Treg cells. However, other studies of HDAC inhibitors provided evidence that Treg cell numbers or function may benefit from HDAC inhibition. Importantly, these alternate suggestions for a mechanism behind HDAC inhibitor-mediated immunosuppression may exist due to the inherent differences present within the HDAC inhibitor classes. The structurally different classes of hydroxamic acids, cyclic peptides, benzamides, epoxyketones, short-chain fatty acids and assorted hybrid molecules all exhibit preferences for different HDAC isoforms [3]. Hydroxamic acids are considered pan-HDAC inhibitors owing to their all-encompassing selectivity. In contrast, benzamides and short-chain fatty acids are only selective for specific isoforms of HDACs [27].