The blank team was presented with sodium chloride injection(0.08 mL·kg~(-1)), even though the design team plus the baicalin team were inserted with 4 mg·kg~(-1) bleomycin. The localization of TGF-β1, mmp2 and timp2 protein in the cells and the mRNA expressions of TGF-β1, mmp2 and timp2 were detected by RT-PCR 14 days later. The results of Biacore affinity analysis showed that the top of binding reaction between Qinbai Qingfei Concentrated Pills and TGF-β1 protein achieved 1 524.0 RU, with specific binding. The affinity constant K_D of baicalin and TGF-β1 was 1.620 06 μmol·L~(-1), that was based on SPR kinetic analysis, suggesting a stable binding between baicalin and TGF-β1, which verified the outcomes of angulation. The results of immunohistochemistry revealed that the deposition of cellulose in baicalin group was less than that in model team, the mRNA expressions of TGF-β1, mmp2 and timp2 had been diminished in baicalin option compared to the design team. Baicalin along with TGF-β1 could restrict the expressions of mmp2 and timp2 and postpone the progress of pulmonary fibrosis.The aim of the report would be to build a rat style of intense pancreatitis(AP) with syndrome of liver despair and Qi stagnation, and supply analysis tools for pharmacodynamic analysis and efficacy community verification of associated traditional Chinese medicine in view of the etiology, pathogenesis and medical manifestations of AP. Based on the Chinese and Western medicine analysis and treatment guidelines for AP with problem of liver despair and Qi stagnation, etiology, pathogenesis and clinical syndromes in TCM, Meta-analysis results, and assessment strategy of setting up an animal model combining disease and syndrome inside our laboratory, the biological surrogate results suited to the evaluation of pet models of AP with syndrome of liver despair and Qi stagnation were removed then. The persistent unpredictable stress(CUS) technique and chronic unstable tension +L-arginine(CUS +L-Arg) technique were used to construct the rat design, plus the preceding biological surrogate outcomes were utilized to evasignificantly increased(P<0.01); the amount of TNF-α mRNA when you look at the pancreatic cells of the CUS group had been significantly increased(P<0.05), but IL-6 mRNA level just revealed a rising trend, suggesting that just the CUS + L-Arg strategy enables you to reproduce the AP harm within the disease-syndrome combo model. The CUS + L-Arg method local antibiotics can be utilized for constant modeling for 30 days to determine a disease-syndrome combo style of AP rats with problem of liver depression and Qi stagnation. The design has the attributes of repeatability, security after mode-ling, reduced pet death, and similar medical pathogenesis. It can be used when it comes to evaluation of old-fashioned Chinese medicine efficacy while the confirmation of effectiveness network.This paper would be to explore the consequence of complete flavonoids of Lichi Semen(TFL) on carbon tetrachloride(CCl_4)-induced liver fibrosis in rats, analyze and predict its system of action and prospective high quality markers(Q-marker). Firstly, male SD rats had been taken and injected subcutaneously with a 40% CCl_4-vegetable oil solution twice per week for 8 successive weeks to establish a rat style of liver fibrosis. The rats with liver fibrosis had been randomly divided in to design group, silybin group(43.19 mg·kg~(-1)), Fuzheng Huayu Capsules group(462.75 mg·kg~(-1)), and TFL groups(100 mg·kg~(-1) and 25 mg·kg~(-1)), with typical rats as a blank group, 10 rats in each team. Except for the empty medical ethics group, the rats in the other teams had been subcutaneously inserted with 40% CCl_4-vegetable oil answer of a maintenance dose, once a week. The rats in various therapy teams got corresponding doses of medicines, while the rats into the empty team and model group received similar level of normal saline daily for four weeks. Atelation with liver fibrosis-related objectives and pathways. Proteomics outcomes indicated that an overall total of 18 proteins among the list of 45 proteins predicted by internet pharmacology had been identified, among which 6 proteins had been considerably expressed, including 5 up-regulated proteins and 1 down-regulated protein. The necessary protein expression of ALB, PLG, HSP90 AA1, EGFR and MAP2 K1 was dramatically gone back to a normal state into the TFL treatment teams. In conclusion, TFL may show the anti-hepatic fibrosis and possible hepatoprotective effects by controlling the phrase of ALB, PLG, HSP90 AA1, EGFR and MAP2 K1, which can be linked to the legislation of multiple signaling pathways related to liver fibrosis such as PI3 K-Akt pathway. Pinocembrin, quercetin, epicatechin, procyanidin A2, naringenin, nobiletin, phlorizin and rutin could be seen as prospective Q-markers of TFL for quality control.Dihydro-β-agarofuran sesquiterpenoids have chemical diversity and biodiversity. A dihydro-β-agarofuran sesquiterpenoid with only hydroxyl groups has-been served by basic hydrolysis of crude extract of Euonymus bungeanus with 0.4% yield. Twelve derivatives were available in esterification, oxidation, decarboxylation, etc. Extensive ~1H-NMR,~(13)C-NMR, MS spectroscopic analyses and single-crystal X-ray diffraction evaluation with Cu Kα radiation indicated that eleven derivatives had been new compounds. The outcomes provides reference for chemistry research on natural item derivatives of dihydro-β-agarofuran sesquiterpenoids.This research is to improve the high quality standard and supply the scientific foundation for Anemarrhenae Rhizoma as well as its raw prepared products. Steroidal saponin including timosaponin BⅡ, timosaponin AⅢ and flavonoids including neomangiferin and mangiferin had been chosen because the click here indicative elements. Silica gel G thin layer chromatography(TLC) and polyamide TLC were used to detect the 2 types of compounds, respectively.