Detection of IL-17A-producing cells was determined by intracellular staining with anti-IL-17-PE (eBio17B7, eBioscience (Frankfurt, Germany)). Foxp3-expressing cells were detected by using the Foxp3 staining kit (anti-Foxp3-PE, FJK, FJK-16s, eBioscience). In some experiments, the amounts of IL-2 secreted by activated cells were measured by ELISA (BD), as described earlier 32. For the IRF-4 immunoblots, whole-cell lysates were prepared as described earlier 32. In brief, phosphatase inhibitors (0.2 mM sodium vanadate, 10 mM sodium fluoride) and 1× complete protease inhibitor (Roche Applied Science) were added into RIPA lysis buffer. Washed cell pellets were incubated on ice for 20 min in RIPA buffer and cell

debris was sedimented by centrifugation at 10 000×g for 10 min. Supernatants Dabrafenib chemical structure were used as cell lysates. The protein concentration was determined using the Micro BCA Protein Assay Kit (Pierce, Rockford, USA) and subsequently 20 μg of total protein were denaturated in 4× Laemmli Buffer and separated by 10% SDS-PAGE. Following SDS-PAGE, samples were transferred to nitrocellulose membrane

(Millipore(Schwalbach am Taunus, Germany)) at 100 V in transfer buffer. For the detection of IRF-4 protein, anti-IRF-4 (M-17, sc6059; Santa Cruz Biotechnology) revealed by donkey anti-goat IgG-HRP (Santa Cruz Biotechnology (Heidelberg, https://www.selleckchem.com/products/AZD2281(Olaparib).html Germany)) was used. As a loading control for protein samples, a monoclonal anti-mouse β-actin antibody (Sigma) was used. For statistical analysis, the two-tailed Student’s t-test was used. Guanylate cyclase 2C p-Values of <0.05 were considered as significant. The authors thank Anna Guralnik and Bärbel Casper for technical support and Hartmann Raifer for helpful discussions. This work was supported by the DFG (SFB TR22, GRK767 and SFB633) and Gemeinnützige Hertie-Stiftung. Conflict of interest: The authors declare no financial or commercial conflict of interest. See accompanying Commentary: http://dx.doi.org/10.1002/eji.201040372 "
“Compounds targeting the chemokine receptor CCR5 have recently been approved for treatment of human immunodeficiency virus (HIV) infection.

Given the central role of CCR5 in inflammation and recruitment of antigen-presenting cells (APC), it is important to investigate the immunological consequences of pharmacological inhibition of CCR5. We evaluated the in vitro effect of different concentrations of CCR5 antagonist maraviroc (MVC) on cell migration of monocytes, macrophages (MO) and monocyte-derived dendritic cells (MDC) towards peptide formyl-methionyl-leucyl-phenylalanine (fMLP) and chemokines regulated upon activation normal T cell expressed and secreted (RANTES) and CCL4/macrophage inflammatory protein-1 (MIP-1β) and CCL2/monocyte chemotactic protein-1 (MCP-1). Results of flow cytometric analysis showed that monocytes treated in vitro with MVC exhibited a significant dose-dependent reduction of chemotaxis towards MIP-1β and MCP-1.