In contrast to CD8(+) T cells, we show that CD4(+) T cells expressing m33 survived for months in vivo. Furthermore, the m33-transduced CD4(+) T cells were able to mediate
antigen-specific rejection of 6-day-old tumors. Together, we show that CD8(+) T cell expressing a MHC class I-restricted high-affinity TCR were rapidly deleted whereas CD4(+) T cells expressing the same TCR survived and provided function while being directed against a class I-restricted antigen. Received 27 June 2011; accepted 1 December 2011; published online 10 January 2012. doi:10.1038/mt.2011.286″
“P>fwa is a late flowering epi-mutant in Arabidopsis thaliana. FWA is silenced by DNA methylation in vegetative tissue but is demethylated in the central cell of the female GSK1120212 ovule and continues to be expressed in the endosperm from the maternal copy. FWA is stably silenced in A. thaliana, but check details in related Arabidopsis species, FWA expression and DNA methylation levels vary in vegetative tissue. In this study, we show that variation in FWA expression in field isolates having identical DNA sequences is associated with changes in DNA methylation
and may change over time. Vegetative FWA expression is correlated with decreased methylation at non-CG sites in the region upstream of the transcription start site in species related to A. thaliana and we conclude that methylation of this region is critical for FWA silencing in these species. In A. thaliana, FWA expression is affected by methylation in regions both upstream and downstream of the transcription start site. Ectopic A. thaliana FWA expression causes a late flowering phenotype,
but over-expression of Arabidopsis lyrata FWA does not. In A. thaliana, stable silencing of FWA to prevent late flowering may have evolved through the selection of large tandem repeats and spread of the critical methylated Metabolism inhibitor region to include these repeats.”
“A computer program has been developed to exploit the multimedia capabilities of a personal computer for a new design of sodar (sonic detection and ranging) data acquisition and control system with minimized hardware elements. Advantages include trouble-free, cost-effective and user-friendly sodar data acquisition using any standard computer. The new design overcomes limitations due to using an add-on data acquisition card with conventional computer-controlled sodar. The data can be processed to produce online display of the dynamics of prevailing atmospheric boundary layer thermal structures and inversion/mixing depth for environmental applications.”
“The present feasibility study evaluated the chorioallantoic membrane (CAM) assay established in cancer and angiogenesis research as a tool for the study of vascular anomalies (VAs) in the head and neck area, since the lack of appropriate model systems poses a major obstacle in VA research.