In this study, Rhodococcus ruber CGMCC3090 is

used for 5-cyanovaleramide (5-CVAM) production by means of adiponitrile (ADN) hydration. Several parameters that affect the biocatalyzation process are investigated. RESULTS: The effective production of 5-CVAM from ADN with good regioselectivity was successfully achieved using the resting cells of Rhodococcus ruber CGMCC3090. The reaction parameters for 5-CVAM production were investigated during the experiment, and it was found that resting cells were effective in converting ADN at high selleck kinase inhibitor concentrations (up to 2.0 mol L-1) and the hydration reaction was slightly inhibited by different concentrations of 5-CVAM. After 100 min of incubation, more than 99.2% of the added adiponitrile was converted to 5-CVAM when the cell concentration was 1.05 g dry cell weight (DCW) L-1. CONCLUSION: The study provides a facile and feasible way of achieving high 5-CVAM production levels while maintaining high purity of the product obtained. Copyright (c) 2012 Society of Chemical Industry”
“BACKGROUND:

Bovine serum albumin (BSA) and Gum Arabic (Acacia) were used to fabricate nanoparticles (NPs) as a new nanocarrier for drug and bioactive delivery systems. Several parameters, namely LB-100 pH, protein to polysaccharide ratio, ionic strength, total biopolymer concentration, temperature, agitation speed and glutaraldehyde concentration were investigated for their effect on the product preparation process. Besides fabrication, the stabilization step including heating, ultrasonicating, cross-linking, and its effect on average size and polydispersity

of NPs was examined. RESULTS: Total biopolymer concentration had no marked effect on the average size of prepared NPs although it did affect other systems produced by complex coacervation. The most effective parameters were HDAC inhibitors in clinical trials pH, temperature and ionic strength, which affected this system (albumin-acacia) by a complicated route. The smallest size of prepared NPs (i.e. the best product) was 108 nm, which was fabricated under the optimum condition of T = 4 degrees C, pH = 4.9, $Pr:Ps = 2$, and Is = 0. CONCLUSION: Of the stabilization methods tested, heating gave the best results. The fabricated NPs were purified with a 0.45 mu m syringe filter and analyzed by PCS, SEM, FTIR and DSC. The mechanism of complex coacervation for preparing NPs, and their use as a nanodelivery system are discussed. Copyright (c) 2012 Society of Chemical Industry”
“BACKGROUND: Many researchers focus on exploring approaches to improve lipid productivity by photosynthetic organisms. Metabolomic analysis provides a new way to globally explore metabolic changes.