It is also possible that mycobacterial infection itself suppresses Th1, IL-17- and IL-22-producing CD4+ T cells or increases Th2 and regulatory T cells, which may limit the protective immune responses. IFN-γ-, IL-17- and IL-22-producing CD4+ T cells in individuals with active TB infection can be induced

by mycobacterial antigens (Fig. 3). Although not significant, a greater number of mycobacteria-specific IL-17- and IL-22-producing CD4+ T cells compared to the unstimulated cells were found in the latent group than in the active TB group. Although more numbers of patients need to be examined, differential IFN-γ, IL-17 and IL-22 responses could potentially improve our ability to distinguish between buy CHIR-99021 latent and active TB infection particularly when a clinical diagnosis is not straightforward [36]. We have shown for the first time that IL-22 is expressed in granulocytes. Interestingly, while intracellular IL-22 protein could be detected, IL-22 mRNA was undetectable in the resting granulocytes. PMA/ionomycin stimulation induced the expression of both IL-22 mRNA as well as intracellular IL-22 protein in granulocytes. The presence of IL-22 selleck products protein in the absence of detectable mRNA is not a unique phenomenon, as other cytokines such as IL-4 [37], IL-8 [38],

macrophage-inflammatory protein 2 (MIP-2) [39], granules and chemokines are also preformed and released rapidly upon stimulation of granulocytes [40,41]. In fact, constitutive expression of MIP-2 mRNA in bone marrow was shown to give rise to peripheral neutrophils with preformed MIP-2 protein [39]. Surprisingly, IL-22-expressing granulocytes in the peripheral blood were found to be higher in healthy controls than in latent TB individuals and even more so in active TB patients. This may be Nintedanib (BIBF 1120) due to localization of IL-22-producing granulocytes in affected

tissues. It is also possible that M. tuberculosis may affect the expression of IL-22 in vivo by inhibiting the synthesis of IL-22. Further studies are needed to investigate IL-22 gene regulation in neutrophils. Although the biological functions of IL-22 have been studied [22,42–45], the regulatory pathway for IL-22 expression is not well characterized. Our preliminary results suggest that neither pathogen-associated molecular patterns including TLR-2, TLR-4 and TLR-9 nor cytokines such as IL-6 and TGF-β, which are known to induce Th17 differentiation [8–10]-induced IL-22 expression in granulocytes (data not shown). We performed comprehensive analysis of a large number of cytokines (IL-1β, IL-2, IL-5, IL-6, IL-8, IL-4, IL-10, IL-12, IL-17, IL-22, IFN-γ, TNF-α and TNF-β) following mycobacterial stimulation of PBMCs and in a set of serum samples from individuals with latent and active TB infection. Our results show clearly that individuals with latent TB infection express differentially a number of proinflammatory and immunoregulatory cytokines.