J Biol Chem 2004,279(24):25066–25074.CrossRefPubMed 51. Dubey AK, Baker CS, Suzuki K, Jones AD, Pandit P, Romeo T, Babitzke P: CsrA regulates ABT-737 translation of the Escherichia coli carbon starvation gene, cstA , by blocking ribosome access to the cstA transcript. J Bacteriol 2003,185(15):4450–4460.PubMedCentralCrossRefPubMed 52. Saitou N, Nei M: The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 1987,4(4):406–425.PubMed 53. Jones DT, Taylor WR, Thornton JM: The rapid generation of mutation data matrices from protein sequences. Comput Appl
Biosci 1992,8(3):275–282.PubMed 54. Lapouge K, Sineva E, Lindell M, Starke K, Baker CS, Babitzke P, Haas D: Mechanism of hcnA mRNA recognition in the Gac/Rsm signal transduction pathway of Pseudomonas fluorescens . Mol Microbiol 2007,66(2):341–356.CrossRefPubMed 55. Lapouge K, Schubert M, Allain FHT, Haas D: Gac/Rsm signal transduction pathway of gamma-proteobacteria: from RNA recognition to regulation of social behaviour. Mol Microbiol 2008,67(2):241–253.CrossRefPubMed 56. Kay E, Dubuis C, Haas D: Three small RNAs jointly ensure secondary metabolism and biocontrol in Pseudomonas fluorescens CHA0. Proc Natl Acad Sci USA 2005,102(47):17136–17141.PubMedCentralCrossRefPubMed buy 4EGI-1 57.
Vodovar N, Vallenet D, Cruveiller S, Rouy Z, Barbe V, Acosta C, Cattolico L, Jubin C, Lajus A, Segurens B, Vacherie B, Wincker P, Weissenbach J, Lemaitre B, Médigue C, Boccard F: Complete genome sequence of the entomopathogenic and metabolically versatile soil bacterium Pseudomonas entomophila . Nat Biotechnol 2006,24(6):673–679.CrossRefPubMed Competing interests We the authors hereby declare that there is no conflict of interests concerning this manuscript.
Authors’ contributions VJC, MV, EA, AV, JMR and FMC conceived the study. VJC and EA did all the cloning and genetics of this study. VJC and MV did the Q-PCR Glycogen branching enzyme experiments and analysis. VJC and JAG did complementation and reporter construct experiments. JMR and AV supported the research. VJC, MV, JMR and FMC wrote the manuscript. VJC, EA, MV, AV, JMR and FMC coordinated and critically revised the manuscript. All authors read and approved the manuscript.”
“Background Escherichia coli O157 (O157) have been implicated in several human outbreaks since their being established as foodborne pathogens in 1982; an estimated 63,153 illnesses, 2,138 hospitalizations and 20 deaths occur annually in the United States [1–4]. Human disease ranges from self-limiting watery diarrhea to debilitating bloody diarrhea that can advance into often fatal, extraintestinal, secondary sequelae in susceptible patients [3, 4]. Cattle are the primary reservoirs for O157, with their recto-anal junction (RAJ) serving as the colonization site at which these human foodborne pathogens persist [4, 5].