N-[5-(Benzylidenamino)-1,3,c-Myc inhibitor 4-thiadiazol-2-yl]sulphonyl benzamide (9a) Offwhitecrystals (EtOH) (this compound was prepared by refuxing 5-amino-1,3,4-thiadiazol-2-[N-(benzoyl)]sulphonamide (2.74 g, 0.01 mol) (4a) and benzaldehyde (8a) (1.06 g, 0.01 mol) in ethanol (20 mL) using 2–3 drops of sulphuric acid as catalyst, for 12 h. It was obtained as yellowish coloured solid and recrystallized by ethanol); yield: 63 %; Mp: 185–187 °C; UV (MeOH) λ max (log ε) 287 nm; R f  = 0.62 (CHCl3/EtOH, 3/1);

FT-IR (KBr): v max 3,625.1, 3,037.4, 1,693.4, 1,678.7, 1,624.32, 1,598.4, 1,557.7, 1,517–1,530.9, SIS3 solubility dmso 1,369.6, 1,290.5, 907.25, 764.44, 756.54, 694.91 cm−1; 1H-NMR (DMSO, 400 MHz): δ = 1.257 (1H, s, –CH–), 2.134 (6H, m, CH–C6H5), 2.590 (6H, m, CO–C6H5), 3.965 (1H, s, CH=N), 4.18 (1H, s, N–H), 7.664–7.685 ppm (10H, m, Ar–H); 13C-NMR ([D]6DMSO, 75 MHz): δ = 171.46 (C, amide), 168.56 (C2, thiadiazole), 166.67 (C5, thiadiazole), 160.68 (C, imine), 137.78 (C1, Ar′–C-imine), 136.05 (C1, Ar–C-amide), 134.24 (C4, CH–Ar′), 132.52 (C3, CH–Ar), 131.71 (C3, CH–Ar′), 130.39 (C5, CH–Ar), 129.29 (C2, CH–Ar′), 129.15 (C6, CH–Ar′), 128.84 (C2, CH–Ar), 128.42 (C6, CH–Ar), 127.34 (C5, CH–Ar′); EIMS m/z [M]+ 370.9 (100);

Anal. for C16H12N4O3S2: C, 51.60; H, 3.25; N, 15.04; S, 17.22. Found: C, 51.61; H, 3.24; N, 15.05; S, 17.22. N-(5-[(4-Chlorobenzylidene)amino]-1,3,4-thiadiazol-2-ylsulfonyl)benzamide (9b) Yield: 64.2 %: Mp: 212–214 °C; PF-6463922 λ max (log ε) 305 nm; R f  = 0.65 (CHCl3/EtOH, 3/1); FT-IR (KBr): v max 3,465.3, 3,417.47, 3,148.51, 1,673.2–1,668.7,

1,624.32–1,598.4, 1,545.9, 1,538.1–1,527.4, 1,368.9–1,358.8, 1,169.9, 968.07, 848–826.5, 764.43–674.43, 764.43 cm−1; 1H-NMR (DMSO, 400 MHz): δ = 1.359 (1H, s, –CH–), 2.342 (6H, m, CH–C6H5), 2.678 (6H, m, CO–C6H5), 3.623 (1H, s, CH=N), 4.41 (1H, s, N–H), 7.462–8.104 (10H, m, Ar–H) 8.24- 8.362 ppm (1H, s, C(=O)N–H); 13C–NMR ([D]6DMSO, 75 MHz): δ = 170.64 (C, amide), 168.41 (C5, thiadiazole), 166.58 (C2, thiadiazole), 161.68 (C, imine), 136.24 (C4, Cl–C–Ar′), 134.16 (C1, Ar–C-amide), 133.78(C1, Ar′–C-imine), 130.25 (C4, CH–Ar), 129.15 (C3, CH–Ar′), 129.29 (C5, CH–Ar′), 129.02 (C3, CH–Ar), 128.97 (C5, CH–Ar), 128.84 (C2, CH–Ar′), 128.42 (C6, CH–Ar′), 127.34 (C2, CH–Ar), 127.29 (C6, CH–Ar); EIMS m/z Tacrolimus (FK506) [M]+ 412.9 (100); Anal.