“
“Objective-To identify factors significantly associated with prognosis in cats hospitalized because of pancreatitis.
Design-Prospective case series.
Animals-33 cats hospitalized for treatment of pancreatitis (diagnosis determined on the basis of clinical signs and serum feline pancreatic lipase immunoreactivity [fPLI] concentration 5.4 mu g/L).
Procedures-Cats were hospitalized (day 1) for 2 to 16 days and observed for 44 days or until they died or were
euthanized. Results of physical Selleck Tariquidar examination and hematologic and serum biochemical analysis, including measurement of serum fPLI concentration, performed on the day of hospital admission were analyzed to determine whether they were associated with outcome (le, survival to at least 44 days vs death or euthanasia).
Results-On day 1, mean +/- SD serum fPLI concentration
among the 33 cats was 22.0 +/- 16.4 mu g/L. Mean age of the cats was 12.7 +/- 3.8 years (range, 4 to 19 years). Eleven of the 33 Galunisertib mw (33%) cats died or were euthanized before day 44. In univariate analyses, dyspnea, hypothermia, hypokalemia, hyperkalemia, and serum fPLI concentration were significantly associated with an adverse outcome. However, in a multivariate analysis, only severe dyspnea, hyperkalemia (potassium concentration > 5.5 mmol/L), and serum fPLI concentration at the time of hospital admission were found to be significantly associated with an adverse outcome.
Conclusions and Clinical Relevance-Results suggested that dyspnea, hyperkalemia, and serum fPLI concentration at the time of hospital admission were significant prognostic factors for cats hospitalized because of pancreatitis.”
“P>A soybean homolog of the tomato FW2.2 gene, here named GmFWL1 (Glycine max FW2.2-like 1), was found to
respond strongly to inoculation with the nitrogen-fixing symbiotic bacterium click here Bradyrhizobium japonicum. In tomato, the FW2.2 gene is hypothesized to control 30% of the variance in fruit weight by negatively regulating cell division. In the present study, the induction of GmFWL1 expression in root hair cells and nodules in response to B. japonicum inoculation was documented using quantitative RT-PCR and transcriptional fusions to both beta-glucuronidase (GUS) and green fluorescent protein (GFP). RNAi-mediated silencing of GmFWL1 expression resulted in a significant reduction in nodule number, with a concomitant reduction in nuclear size and changes in chromatin structure. The reduction in nuclear size is probably due to a change in DNA heterochromatinization, as the ploidy level of wild-type and RNAi-silenced nodule cells was similar. GmFWL1 was localized to the plasma membrane. The data suggest that GmFWL1 probably acts indirectly, perhaps through a cellular cascade, to affect chromatin structure/nuclei architecture. As previously proposed in tomato, this function may be a result of effects on plant cell division.