P. acnes isolates differ in

their virulence properties, such as in their ability to trigger production of proinflammatory cytokines/chemokines in infected keratinocytes [21, 22]. The genetic basis for this has not yet been studied in detail. To date four phylogenetic groups of P. acnes have been described, designated types IA, IB, II and III, based on sequence differences in two genes, namely recA and tly [23, 24]. Despite the apparent role of P. acnes in disease formation, information on putative pathogenic traits and antigenic substances of this bacterium is scarce. The complete genome sequence of a cutaneous type IB https://www.selleckchem.com/products/Trichostatin-A.html isolate of P. acnes (strain KPA171202) provided insights into the pathogenic potential of P. acnes, revealing 3-Methyladenine in vitro numerous gene products SB-715992 in vitro with putative host tissue-degrading activities as well as predicted cell wall-associated and secreted proteins, the presence or activity of which might be involved in triggering host tissue inflammation [25]. Some of these proteins are differentially expressed among P. acnes isolates and were shown

to be immunoreactive [26]. To shed light on the biological relevance of predicted genes from the genome sequence, we used a combination of two-dimensional electrophoresis (2-DE) and matrix-assisted-laser-desorption/ionization mass spectrometry (MALDI-MS) to identify proteins secreted by P. acnes. Isolates representing all four phylotypes were investigated. Several hydrolases and putative virulence factors were secreted by all strains tested. These factors are potential host-interacting factors, likely important in inflammatory responses to P. acnes, as observed in acne vulgaris. Thus, our data provide a basis to guide further in-depth studies on individual factors. Results and Discussion Choice of P. acnes strains We selected five strains of P. acnes for analysis of their secreted proteins. These strains, representing all known P. acnes phylotypes, i.e. types IA, IB, II and

III, were isolated from a range of tissue click here sites: a type II skin acne isolate (strain 329); a type III strain isolated from a post-operative prosthetic joint infection (strain 487); a type IA strain isolated from a pleuropulmonary infection (strain 266); and two type IB strains: a skin isolate for which the genome sequence is available (strain KPA171202; KPA); and an isolate from a cancerous prostate (strain P6). 2-DE-MALDI-MS analysis of P. acnes culture supernatants To identify proteins secreted by P. acnes using proteome analysis, we cultured each strain under anaerobic conditions in brain heart infusion (BHI) broth, previously used for secretome analyses [27]. Growth curves were generated (data not shown) and culture supernatants were harvested in the mid-exponential phase. Precipitated proteins from supernatants were separated by 2-DE and Coomassie stained, generating reproducible secretomes of all five strains tested (Fig. 1A-E and additional file 1).