Stromata in 3% KOH after rehydration tubercular and darkening, without a conspicuous colour change. Stroma anatomy: Ostioles (67–)75–110(–117) μm long, plane or projecting to 15(–20) μm, (22–)25–40(–45) μm wide at the apex (n = 15), cylindrical or conical, periphysate, with apical palisade of inconspicuous, hyaline, narrowly clavate cells. Perithecia (135–)170–250(–265) × (130–)160–250(–285) μm (n = 20), globose. Peridium (12–)15–21(–25) μm thick at the base and sides (n = 40). Cortical layer (17–)20–30(–35) μm (n = 30) thick, surrounding

the entire stroma except the area of attachment, an orange-brown t. angularis of indistinct cells (3–)4–9(–12) × (2.5–)3–7(–11) μm (n = 60) in face view and in vertical section, with inhomogeneous pigment distribution; cells more distinct in vertical section. Hairs on mature stroma (7–)10–23(–26) × (2.0–)2.5–3.5(–4.0) TGF-beta inhibitor μm (n = 20), cylindrical, simple or sparsely branched, with narrowly

rounded ends. Subcortical tissue a t. intricata reaching to the base of the perithecia, of thin-walled hyphae (2.2–)3.3–5.5(–5.7) μm (n = 20) wide, partly appearing as t. globulosa due to variable orientation of hyphae. Subperithecial tissue a t. angularis of hyaline, partly brownish cells (5–)7–18(–23) × (4–)6–14(–20) μm (n = 30). Asci (74–)78–89(–94) × (4.8–)5.0–5.8(–6.2) μm, Erismodegib cost including selleck chemicals a (5–)7–13(–16) μm long stipe (n = 30). Ascospores hyaline, verrucose, cells dimorphic, but often of similar shape, distal cell (3.4–)3.8–4.5(–5.3) × (3.3–)3.7–4.4(–4.6) μm, l/w 1.0–1.1(–1.2), (sub)globose, proximal cell (3.3–)4.0–5.4(–6.2) × (2.7–)3.0–3.7(–4.2) μm, l/w (1.0–)1.1–1.7(–2.3) (n = 30), oblong, plump wedge-shaped or subglobose. Cultures and anamorph: optimal growth at 25°C on all media; hyphae dying after short and limited growth at 35°C. On CMD after 72 h 26–29 mm at 15°C, 45–48 mm at 25°C, 38–42 mm at 30°C, <1 mm at 35°C; mycelium covering the plate after 5 days at 25°C. Colony hyaline, thin; mycelium Tangeritin loose, reticulate, denser at the wavy, ill-defined margin;

hyphae with little variability in width. Aerial hyphae inconspicuous, becoming fertile. No autolytic excretions, no coilings seen. No pigment noted, odour coconut-like. Chlamydospores noted after 1 days, after 11 days numerous, particularly close to conidiation tufts, (7–)8–10(–11) × 7–9(–10) μm, l/w (0.9–)1.0–1.1(–1.3) (n = 30), globose or ellipsoidal, mostly terminal, smooth. Conidiation noted after 2 days, grey- to dark green, 26DE4–6, 26F5–8, after 3 days, in fluffy tufts or loose pustules 0.5–2(–4) mm diam with irregular or circular outline, arranged in several indistinctly separated, concentric zones, irregularly confluent to 7 mm. Tufts arising on thick-walled, verrucose 6–19 μm wide stipes, branching asymmetrically into primary branches of similar width, rebranching mostly at right angles.