The patients’ weights were measured by Seca scale with 0.1 kg accuracy. Also, using a questionnaire, data were collected on age, literacy level, occupation, type of therapy for diabetes, and type of delivery using medical history and interview with the patients. Laboratory Measurements After

an overnight fasting, samples of 6 ml peripheral blood were taken from all of the patients at most 10 days after the delivery, and were used to determine plasma glycosylated hemoglobin A1C (HBA1C) test by immunoassay method. Also, blood samples were used to Rapamycin cell line prepare serums, which were Inhibitors,research,lifescience,medical kept frozen at -80°C until analysis. Fasting blood samples were also obtained on follow ups and were used to prepare serums, which were kept similarly. The serum samples were used to measure 25(OH) vitamin D3, paratormone (PTH), calcium and phosphorus. The serum 25(OH) vitamin D3 was measured by ELISA and kit of immunodiagnostic systems Ltd (Nyco card equipment, Nyco corporation, Norway). The sensitivity

Inhibitors,research,lifescience,medical of the test was 2 nmol/ml. Serum paratormone was also measured by ELISA and immunodiagnostic systems Ltd (IDS Ltd), which had a sensitivity of 0.6 picomol/l. The serum calcium and phosphorus were respectively measured by calorimetric method by AutoAnalyzer (Echoplus Corporation, Italy), Biosystems kit (Spain), and ELISA method. Dose and Follow-Up The administered Inhibitors,research,lifescience,medical vitamin D was vitamin D3, which were kept from light, frozen, and stored at 15 to 30oC during the study. Patients’ Inhibitors,research,lifescience,medical vitamin D status was determined by measuring serum 25(OH) vitamin D3 concentration. Serum concentrations of lower than 12.5 nmol/l was considered severe deficiency, 12.5 to 24.9 nmol/l was taken as moderate deficiency, 25 to 34.9 nmol/l as mild deficiency, and concentrations higher than 35 nmol/l was regarded as optimal.12 Statistical Analysis The data were

analyzed by the SPSS package Version 11 (SPSS Inc., Chicago, IL, USA). Kolmogro-Smirnov test was used to determine the distribution of quantitative data. Between-group Inhibitors,research,lifescience,medical comparisons were made using t or Wilcoxon tests, and within-group comparisons were made using paired t-test second or Mann-Whitney U-tests. Between-group and within-group comparisons of qualitative data were performed Chi-Square or McNemar tests. A P value of ≤0.05 was considered statistically significant. Results Forty five patients including 24 with the age of 30.7±6.2 years in the IG and 21 with the age of 29.5±4.0 years in the CG completed the study. There was no significant difference between the body mass index (BMI) for the subjects in the IG (28.9±4.8 kg/m2) and CG (27.9±3.6 kg/m2) (table 1). Moreover, there was no significant difference between the two groups in terms of plasma HA1C, literacy level, type of treatment for GDM, or type of delivery.