The selection of a suitable purging time was based Navitoclax mw on the desired sensitivity

for the analyzed tracers (nature of sample). Short purging time increased the analytical sensitivity to isoprene, DMS and benzene while longer purging time increased the sensitivity to toluene, xylenes and α-pinenes. In our case, ocean samples were analyzed. DMS and isoprene are known ocean emissions (17–34 Tg S/yr (Carpenter et al., 2012 and Spielmeyer and Pohnert, 2012) and 1–11.6 Tg C/yr (Arnold et al., 2009, Carpenter et al., 2012 and Shaw et al., 2010) accordingly) while tracers like α-pinenes have been reported only rarely in the marine environment (first reference (Yassaa et al., 2008)) and therefore concentrations were expected to be low. Taking this into consideration, a purging time of 10 min (400 ml) was chosen here as a good compromise for all investigated FK506 cell line tracers. The method was evaluated using the selected purging volume providing good sensitivity and reproducibility for all examined tracers (see Section 3.1, Method evaluation). Seawater and calibration standard samples were analyzed immediately after sampling. The NTDs were thermally desorbed in the injection port of the GC. The injector temperature was set to 310 °C to ensure complete and fast desorption. As shown, in a previous study

(Trefz et al., 2012), a temperature of 290 °C or higher is recommended in order to achieve complete desorption and negligible carry over for needle traps containing PDMS as a sorbent material. The whole length of the needle was inserted into the GC injector through a Merlin microseal septum while the Luer lock end of the needle remained sealed with a Teflon cap. Desorption was achieved in split-less mode of the GC injector for 30 s. Rapid introduction of analytes into the column was accomplished through the narrow glass liner. The temperature of the GC-column was maintained at 40 °C for 5 min, then increased to 95 °C at 1.5 °C per min and held at this temperature for the rest Thymidylate synthase of the analysis. Helium 6.0 was used as carrier gas at a flow rate of 0.8 ml/min. The mass spectrometer (MS), with an electron

impact source running in SIM mode, was operated with the following conditions: ionization potential of 70 eV and source temperature of 230 °C. The examined compounds were separated into five groups where for each compound a dwell time of 100 ms was applied. In this way, clean (artifact peak free) chromatograms were obtained with high sensitivity for each compound. The SIM parameters used are presented in Table 1. After analysis, the column temperature and flow were slightly increased for a few minutes (above 100 °C) so that any water remaining in the column would be purged from the system and not affect the subsequent analysis. The above settings provided sharp, reproducible peaks and good separation for all examined compounds within 23 min.