The sequence was assembled in Bionumerics version 4.0 (Applied Math, Sint-Martens-Latem, Belgium) and checked for chimeras both by blasting the individual sequences in GenBank http://www.ncbi.nlm.nih.gov and by the software Pintail version 1.1 http://www.cardiff.ac.uk/biosi/research/biosoft/. The phylogenetic analysis of the clones belonging to the Escherichia genus was done by downloading 16S rRNA gene sequences longer than 1,200 bp from the
RDP v.9 database of the Escherichia type strains http://rdp.cme.msu.edu. The sequences were trimmed to the same length of 1327 bp and aligned pairwise (UPGMA) followed by a global sequence alignment. A final phylogenetic tree was constructed by using the WARD algorithm where Enterobacter selleck chemical sakazakii (AB004746) was used as outgroup. Acknowledgements
The authors wish to thank Hanne H. Møller, Katja Kristensen and Johanna Z Amenuvor for technical assistance in the laboratories. Also thanks to Stina Vesterholm for helping collecting tissues. This work was supported by Kongeriget Danmark’s Horseinsurance g/s and Intervet Denmark. Sponsors had no involvement in the practical part or conclusions of this study. References 1. Lorenzo-Figueras M, Merritt AM: Effects of exercise on gastric volume and pH in the proximal portion of the stomach of horses. Am J Vet Res 2002, 63:1481–1487.PubMedCrossRef 2. Murray MJ, GSK2118436 chemical structure Nout YS, Ward DL: Endoscopic findings of the gastric antrum and pylorus in horses: 162 cases (1996–2000). RVX-208 J Vet Intern Med 2001, 15:401–406.PubMed 3. Begg LM, O’Sullivan CB: The prevalence and distribution of gastric ulceration in 345 racehorses. Aust Vet J 2003, 81:199–201.PubMedCrossRef 4. De Groote D, Van Doorn LJ, Van den BK, Vandamme P, Vieth M, Stolte M, Debongnie JC, Burette A, Haesebrouck F, Ducatelle R: Detection of non-pylori Helicobacter
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