Their uses are increasing world wide due to the persistent and sometimes expansion of traditional medicine

and a growing interest in herbal treatments.1 Inflammation is part of the complex biological response of vascular tissues click here to harmful stimuli including pathogens, irritants or damaged cells.2 It is also a pathophysiological response of living tissues to injuries that leads to the local accumulation of plasmatic fluids and body cells. It is a protective attempt by an organism to remove injurious stimuli as well as initiate a healing process for tissues. The process of inflammation is necessary for healing of wounds, however, if not controlled, may lead to the onset of diseases as vasomotor rhinorrhoea, rheumatoid arthritis, atherosclerosis and cancer inter alia.3 Alstonia boonei

de Wild ( Fig. 1) (Apocynaceae) is a medicinal plant used extensively in west and central Africa. It has been found to elicit several pharmacological and therapeutic actions. It is a large deciduous tree that is up to 45 m tall and 1.2 m in diameter; bole often deeply fluted up to 7 m; small buttresses present; bark greyish-green or grey; rough, exuding a copious milky latex and branches in whorls. It occurs from Senegal and Gambia to Western Ethiopia and Uganda where it is found Autophagy Compound Library screening in primary as well as secondary moist evergreen to dry semi-deciduous forest. In west and central Africa, its parts are generally used for the treatment of many ailments including malaria, fever, intestinal helminths, rheumatism,

hypertension and other life-threatening diseases. 4 An infusion of the root and stem bark is drunk as a remedy for asthma; a liquid made from the stem bark and fruit is drunk once daily to treat impotence. 5 Other reported properties of A. boonei include: anti-viral, anti-microbial and antioxidant activities. 6 This study was aimed at investigating the effect of the ethanol extract of the stem bark of A. boonei on leucocyte migration in Wistar rats. Stem bark of A. boonei tree was collected from the Botanical Garden of the University of Nigeria, Nsukka, Enugu State, Olopatadine Nigeria. The botanical identification of the stem bark was done by Prof. (Mrs.) May Nwosu of the Department of Botany, University of Nigeria, Nsukka. Fresh stem bark of A. boonei tree was washed with distilled water and cut into smaller bits to increase their surface area for easier drying. The stem bark was shade-dried for a month and a half and homogenised into fine particles using an electric blender. A known weight (372 g) of the ground stem bark was macerated in 1500 ml of 80% ethanol for 24 h at room temperature. The mixture was filtered and the filtrate passed through a rotary evaporator to reduce the ethanol content. Thereafter, the filtrate was further concentrated using an oven at 50 °C and stored in a refrigerator until used.