With
the Mantel-Haenszel algorithm, we tested the CDK4 IVS4-nt40 G→A genotype variant for association with cancer and with EPZ015666 ic50 tumors/cancer against control subjects with no cancer and no tumors/cancer, respectively. We further tested the CDK4 IVS4-nt40 check details G→A at genotype level for association with obesity-associated cancer and with obesity-associated tumors/cancer against non-obese control subjects with no cancer and no tumors/cancer, respectively. We also perfomed an association test for non-obese cancer and tumors/cancer cases. Results All alleles tested in each group of the four datasets were not in departure from HWE. We did not identify in our dataset any significant and valid association of the CDK4 IVS4-nt40 G→A genotype variant Ferrostatin-1 with either cancer or tumors/cancer against control subjects with
no cancer and no tumors/cancer, respectively (Table 3, 4). However, our dataset may not be able to detect any risk variant with a modest effect contributing to cancer and/or tumors/cancer. Table 3 CDK4 IVS4-nt40G→A genotype association with cancer Genotype 46 cancer 204 No cancer X2 2-t P OR 95% C.I. + – + – AA 7 39 14 190 3.405 0.060 2.44 0.83 – 7.00 AG 20 26 76 128 0.615 0.433 1.30 0.64 – 2.60 GG 19 27 114 90 3.204 0.073 0.56 0.28 – 1.11 X2 = Chi-Square, 2-t P = 2-tailed p-value, OR = odds ratio, C.I. = confidence interval Table 4 CDK4 IVS4-nt40G→A genotype association with tumor/cancer Genotype 152 Tumor/cancer 111 No tumor/cancer X2 2-t P OR 95% C.I. + – + – AA 19 133 6 105 3.754 0.053 2.50 0.90 – 7.28 AG 57 95 52 59 2.309 Rucaparib 0.129 0.68 0.40 – 1.15 GG 76 76 53 58 0.130 0.718 1.09 0.65 – 1.84 X2 = Chi-Square, 2-t P = 2-tailed p-value, OR = odds ratio, C.I. = confidence interval In the subset of the obesity-associated tumor/cancer analysis, we identified a significant
association of the CDK4 IVS4-nt40 AA genotype with BMI ≥ 30 and cancer (P = 0.002, Table 5), and with BMI ≥ 30 and tumors/cancer (P = 0.007, Table 6). We had in our datasets of genotype association tests with the obesity-associated cancer and obesity-associated tumors/cancer at least 60% power to detect the identified risk ORs identified (Table 2). The analysis performed to exclude association of the CDK4 IVS4-nt40 AA genotype with the subset of non-obese cancer and tumors/cancer was not significant (data not shown). Table 5 CDK4 IVS4-nt40G→A genotype association with cancer and BMI ≥ 30 Genotype 10 Cancer and BMI ≥ 30 178 No cancer and BMI<30 X2 2-t P OR 95% C.I. + – + – AA 3 7 9 169 9.858 0.002 8.05 1.37 – 44.21 AG 2 8 66 112 1.196 0.274 0.42 0.06 – 2.25 GG 5 5 103 75 0.240 0.624 0.73 0.17 – 3.03 BMI = body mass index, X2 = Chi-Square, 2-t P = 2-tailed p-value, OR = odds ratio, C.I.