Glossary Abbreviations AP acute pancreatitis MNL monomorphonuclear leucocytes MPO myeloperoxidase PMNL polymorphonuclear leucocytes RIA radioimmunoassay ROS reactive oxygen species TAP trypsinogen activation peptide Conflict of interest The authors state no conflict of interest. Supporting information Additional Supporting Information may be found in the online especially version of this article: Figure S1 (A) mRNA and (B) protein expression of CD11a mRNA in peripheral neutrophils from wild-type (WT) and lymphocyte function antigen-1-deficient (LFA-1?/?) mice. ��-Actin serves as a housekeeping gene. The results presented are from one experiment, which is representative of four others. Click here to view.(150K, ppt) Video S1 Intravital fluorescence clip of a postcapillary venule in a) wild-type and b) lymphocyte function antigen-l-deficient mouse.
Pancreatitis was induced by infusion of sodium taurocholate into the pancreatic duct. The video was recorded 24 h after induction of pancreatitis. Click here to view.(4.9M, mpg) Click here to view.(4.9M, mpg) Video S2 Intravital fluorescence clip of capillaries in a wild-type mouse. Pancreatitis was induced by infusion of sodium taurocholate into the pancreatic duct. The video was recorded 24 h after induction of pancreatitis. Click here to view.(5.9M, mpg) Click here to view.(738K, pptx) Please note: Wiley-Blackwell are not responsible for the content or functionality of any supporting materials supplied by the authors. Any queries (other than missing material) should be directed to the corresponding author for the article.
In the present study we analyzed immunohistochemical expression of MAGE-A 3/4 and NY-ESO-1 in 55 samples of esophageal squamous cell carcinomas (ESCC) and their respective lymph node metastases. To our knowledge this is the first study to assess and compare the expression of these antigens in ESCC lymph node metastases. Fifty (90.9%) primary ESCC were positive for MAGE-A 3/4 and 53 (96.6%) were positive for NY-ESO-1. MAGE-A 3/4 was expressed in all lymph node metastases and the intensity of expression was high in a majority of cases. NY-ESO-1 was negative in 2 (7.1%) lymph nodes metastases, while the reaction was predominantly moderate in the positive group. In primary tumors MAGE-A 3/4 showed a significantly higher intensity of expression compared to NY-ESO-1 (P=0.
047), while in lymph node metastases the intensity of expression was not significantly different (P=0.387). Primary tumors with and without lymph node metastases showed no significant differences in MAGE-A 3/4 (P=0.672) and NY-ESO-1 (P=0.444) expression. Intensity of MAGE-A 3/4 (P=0.461) and NY-ESO-1 (P=0.414) expression in primary tumors was not significantly different compared to the expression Brefeldin_A in their respective lymph nodes metastases. Expression of MAGE-A 3/4 in primary tumors showed significant positive correlation with primary tumor expression of NY-ESO-1 (P=0.