Key Word(s): 1. cancer stem cells; 2. Lgr5 and CD44; 3. colon polyps; 4. colorectal cancer; Presenting Author: HONG WEI Additional Authors: XIN-PU MIAO Corresponding Author: HONG WEI Affiliations: Department of GastroenterologyHai Nan Provincial People’s Hospital Objective: To study the detection and clinical ABT-199 significance of blood

platelets count and Coagulation in patients with ulcerative colitis (UC). Methods: The levels of peripheral blood platelets count (BPC) and coagulation in patients with UC (n = 57) and normal control group (n = 26) were detected and the effects on disease severity were analyzed subsequently. Results: The levels of peripheral BPC and FIB in active phase group were significantly higher than those in control group (P < 0.01), PT in active phase group were significantly lower than those in control group (P < 0.01);

the levels of peripheral blood platelets count (BPC) and FIB in severe stage were significantly higher than those in patients medium and mild stage, PT in severe stage were significantly lower than those patients in medium and mild stage (P < 0.01). Blood platelets Selleckchem MDV3100 count (BPC) were correlated with FIB in patients with UC, and were negative correlated with PT. Conclusion: I t is proposal that blood platelets count and Coagulation would provide useful marker of active of UC, They had important value to judge active phase and severity of UC. Key Word(s): 1. Ulcerative colitis; 2. Blood platelets; 3. Coagulation; Presenting Author: FEIXUEFEI CHEN Additional Authors: YANBOYAN BO, XIULI ZUO, YANQING LI Corresponding Author: XIULI ZUO Affiliations: no Objective: As a member of the nerve growth factor family, brain-derived neurotrophic factor (BDNF), widely distributed in the central central, peripheral and enteric

nervous system, plays fundamental roles in the differentiation, survival and maintenance of neurons. Besides these roles, BDNF has been implicated to enhance gastrointestinal motility. There are growing evidences to support this view. Patients with a variety of neurologic disorders or ALS who were MCE treated with r-metHuBDNF appeared to have a dose-related “diarrhea.” A 2-week treatment with recombinant BDNF dose-dependently accelerated colonic emptying and increased stool frequency. In rats, BDNF has an excitatory effect on myoelectric activities of the colon. Endogenous BDNF enhances the peristaltic reflex by augmenting the release of serotonin and calcitonin gene-related peptide induced by mucosal stimulation. All these suggest that BDNF plays an important role in gut motor functions. However, there are no researches about the acute and direct effects of BDNF on the contractile activity in the isolated intestinal tracts of mice. In central, Binding of BDNF to TrkB dimers activates the phospholipase C (PLC) pathway, which results in the formation of the second messengers DAG and IP3 and calcium release from intracellular stores.