Significance and Impact of the Study:
The Dot-ELISA test is simple, specific, rapid and inexpensive. It is suitable for identifying a large number of samples and, hence, reducing the death rate of patients with leptospirosis.”
“Aims:
To study the diversity of rumen methanogens in Murrah buffaloes (Bubalus bubalis) from North India by using 16S rRNA gene libraries obtained from the pooled rumen content from four animals Idasanutlin molecular weight and using suitable software analysis.
Methods and Results:
Genomic DNA was isolated and PCR was set up by using specific primers. Amplified product was cloned into a suitable vector and the positive
clones were selected on the basis of blue-white screening and sequenced. The resulting nucleotide sequences were selleck inhibitor arranged in the phylogenetic tree. A total of 108 clones were examined, revealing 17 different 16S rRNA gene sequences or phylotypes. Of the 17 phylotypes, 15 (102 of 108 clones) belonged to the genus Methanomicrobium, indicating that the genus Methanomicrobium is the most dominant component of methanogen populations in Murrah buffaloes (Bubalus bubalis) from North India. The largest group of clones (102 clones)
was more than 98% similar to Methanomicrobium mobile. BLAST analysis of the rumen contents from individual animals also revealed 17 different phylotypes with a range of 3-10 phylotypes per animal.
Conclusion:
Methanomicrobium phylotype is the most selleck screening library dominant phylotype of methanogens present in Murrah buffaloes (Bubalus bubalis).
Significance and Impact of the Study:
Effective strategies can be made to inhibit the growth of Methanomicrobium phylotype to reduce the methane emission from rumen contents and thus help in preventing global warming.”
“Aims:
The goal of the study was to develop a reliable, reproducible and rapid method of culture in order to screen a large number of fungal transformants.
Methods and Results:
The method is based upon miniaturized cell cultures and automated expression screening in microwell plates. For the method
development, 50 recombinant Aspergillus vadensis clones producing feruloyl esterase B (FaeB) from Aspergillus niger were screened in 6 days. Then a panel of clones showing various behaviours was checked in flasks in order to demonstrate the reproducibility of the method. Using this method, a transformant of A. vadensis producing 1 center dot 2 g l(-1) of FaeB was selected (12-fold more than the A. niger overproducing strain).
Conclusions:
This miniaturized culture method allows to obtain reliable and reproducible results. The procedure has the advantages of being efficient, time-saving and more efficient than conventional in-flask culture screening as it can screen 800 clones per day after a culture of 3 days.
Significance and Impact of the Study:
This method could be applied to any other fungal strain culture, enzyme activity or biodiversity screening.