In addition to mbhA, several intercellular genes (including asgA

In addition to mbhA, several intercellular genes (including asgA and popC) JQ1 research buy appear to have been subject to HGT (Goldman et al., 2007), but all of the intracellular pathway genes assessed by Goldman et al. (2007) seem to have evolved vertically. Firstly, on average, intercellular genes have more severe phenotypes upon deletion than intracellular genes (Fig. 2), producing approximately fivefold fewer spores (14% and 72% of wild-type sporulation, respectively). Secondly, intracellular genes are more variable than intercellular genes, as manifested

by lower mean percentage identities and similarities when aligned against their orthologues in S. aurantiaca (67% identity and 78% similarity compared with 77% identity and 85% similarity, respectively). There is a medium strength correlation (ρ=0.374) between percentage identity and percentage of wild-type sporulation. Developmental timers

and nutrient sensors also differ quantifiably. Developmental timers have a small average effect on spore yield upon deletion (117% of wild-type sporulation) and high sequence variability (61% identity and 75% similarity to S. aurantiaca orthologues), whereas nutrient sensors have relatively severe effects on deletion (44% of wild-type sporulation) and exhibit reduced find more sequence variability (72% identity and 81% similarity to their S. aurantiaca orthologues), as can be seen in Fig. 2. Intracellular pathway genes were found on average to lie only 1374 coding sequences (CDSs) from the origin (17.3% of the genome), while the mean for intercellular pathway genes was 2106 CDSs (27.0% Docetaxel of the genome). The average for all genes in the genome is 1879 CDSs (25% of the genome). Developmental timer genes were found to lie particularly close to the origin (mean 628 CDSs, 7.8% of the genome), while nutrient sensor genes averaged 1891 CDSs from the origin (23.6% of the genome). Genomic location and sequence conservation (percentage identity) exhibit a medium strength correlation (ρ=0.428), while the genomic location and severity of a phenotype are strongly correlated (ρ=0.651). Student’s two-sample t-tests (not assuming

equal variance) lent highly significant support (P<0.05 in all cases) to the proposal that the intercellular and intracellular genes assessed had been sampled from discrete populations, whether assessing percentage identity, percentage similarity, distance from origin or severity of phenotype. Statistically significant correlations were also observed between genomic location, sequence conservation and severity of phenotype (reported above), and correlation coefficients were of a similar magnitude whether derived from parametric or nonparametric (Spearman) tests of correlation. Further support for categorization on the basis of a mechanistic role (intercellular vs. intracellular, and nutrient sensor vs. developmental timer) was also obtained from a variety of nonparametric tests, including Mann–Whitney U-tests (P<0.

parahaemolyticus of clinical and environmental origins PCR metho

parahaemolyticus of clinical and environmental origins. PCR methods have been applied FK506 nmr to the detection of bacterial pathogens for decades (Bej et al., 1999; Liu et

al., 2004a, 2005; Bauer & Rorvik, 2007; Kim et al., 2008a). The specificity of target sequences is crucial for their accurate identification. Specific genes or universal genes, including toxin genes and 16S rRNA gene, have been used as target markers for PCR assays (Martinez-Picado et al., 1994; Bej et al., 1999). Unfortunately, there is often significant nucleotide sequence similarity among toxin genes in bacterial species, especially within the same genus, and this sequence similarity has prevented these toxin genes from being useful targets for species-specific identification of bacterial pathogens (Chizhikov et al., 2001). The 16S rRNA gene sequences among the Vibrionaceae family showed >90% nucleotide sequence similarity when www.selleckchem.com/products/LBH-589.html analyzing this gene of 35 Vibrio strains (Urakawa et al., 1997). It seems that the high degree of sequence identity does not allow reliable discrimination of specific strains using PCR methods. Computational genomics has led the way to efficient and customized mining of genomes for species-specific nucleotide sequences. The blast program, a frequently used tool for nucleotide sequence

comparisons, has been applied to identify specific targets for the detection and identification of bacterial pathogens (Oggioni & Pozzi, 2001; Kim et al., 2006, 2008b). To mine targets with a high level of specificity, we identified 23 V. parahaemolyticus-specific candidate CDSs by standalone blast searching against the local database. Among the 23 V. parahaemolyticus-specific candidate CDSs, seven were designated hypothetical proteins, 14 were identified as putative genes and two were characterized by their function. Revealing the specificity of CDSs might be helpful in understanding the metabolic behaviors unique to V. parahaemolyticus. The specificity in silico is largely determined

Olopatadine by the screening criteria. If blastn searching of a query sequence returns a best-match sequence with the lowest e-value ≥0.001, the query sequence is considered to share little or no sequence similarity to any nucleotide sequence in the database, and, for our purposes, should be considered a specific sequence target (LaGier & Threadgill, 2008). Here, we chose the lowest e-value ≥0.1 as a standard to select V. parahaemolyticus-specific CDSs. In general, the process of identifying specific sequences will be made more reliable by the addition of more bacterial genomes to the database used for blast comparison. In this study, genome sequences of 811 non-V. parahaemolyticus bacteria proved to be sufficient for identifying V. parahaemolyticus-specific CDSs.

VC-M is supported

VC-M is supported www.selleckchem.com/products/jq1.html by a fellowship from the JdlC programme and grant JCI-2010-06395. XE is supported by a fellowship from the JdlC programme and grant JDCI20071020. The constructive comments and criticisms of the two anonymous reviewers helped us to improve the manuscript and are greatly appreciated. Conflicts of interest: The authors declare no competing interests. Other members of the HIV Lipodystrophy

Study Group and contributors to this paper are: Verónica Alba, Alba Aguilar, Teresa Auguet, Matilde R. Chacón, Miguel López-Dupla, Anna Megia, Merce Miranda, Montserrat Olona, Amadeu Saurí, Montserrat Vargas, Ignacio Velasco and Sergi Veloso (Hospital Universitari Joan XXIII, IISPV, Universitat Rovira i Virgili, Tarragona, Spain); Àngels Fontanet, Mar Gutiérrez, Gràcia Mateo, Jessica Muñoz, Ma Antònia Sambeat (Hospital de la Santa Creu

i Sant Pau, Universitat Autònoma de Barcelona, Barcelona, Spain). “
“Prospective pharmacogenetic screening for the human leucocyte PLX4032 antigen (HLA) B*5701 allele can significantly reduce the number of cases of abacavir-related hypersensitivity among HIV-infected patients treated with this drug. The aim of this study was to establish the frequency of the HLA B*5701 variant in HIV-infected Poles. The sequence-specific primer (SSP) test was used to assess the feasibility of the introduction

of such testing in clinical practice. Progesterone For this purpose, 234 randomly selected HIV-positive patients were screened using a low-resolution SSP assay, with HLA B*5701-positive results confirmed using a high-resolution test. The HLA B*5701 variant was found in 11 of 234 subjects (4.7%). Testing with the selected method proved quick and reliable. Despite extensive research in the field of pharmacogenetics, routine genetic marker testing for clinical purposes is not common. One successful example of the implementation of such a test into practice is human leucocyte antigen (HLA) B*5701 testing among people living with HIV, prior to the introduction of the nucleoside reverse transcriptase inhibitor abacavir to antiretroviral treatment. The drug was associated with hypersensitivity reactions (HSRs), which were noted in up to 8% of Caucasian individuals after challenge with the drug [1]. Hypersensitivity can occur within 6 weeks of treatment initiation and most commonly manifests clinically as fever, rashes, respiratory and gastrointestinal symptoms or malaise/lethargy [2]. The symptoms resolve quickly, within 72 hours of drug discontinuation. Re-challenge with the drug in hypersensitive individuals can be fatal, with acute anaphylaxis and hypotension [3].

On the other hand, despite a high number of isolates, no strain i

On the other hand, despite a high number of isolates, no strain isolated from clam haemolymph demonstrated antibacterial activity. The target bacteria spectrum and/or the growth conditions [nutrients (Spanggaard et al., 2001) and/or temperature (Zhang et al., 2012) or bacterial presence in the surroundings (Mearns-Spragg et al., 1998; Dusane et al.,

2011)] may explain these results. Nonetheless, the potential of bivalve microbiota as a source of antimicrobial compounds is evident, although underexplored. The cryogenic storage resulted in total loss of cultivability for five strains (hMe-15, -22, -82, -119 and -131) and the cell-free supernatant of a further nine strains did not exhibit antibacterial PF-01367338 cost activity (hCg-60, -78, -111 and-114, hPm-100 and -102, hMe-34, -43 and -273). Such loss of cultivability or bioactivity after storage is frequently described with marine bacteria (Gram et al., 2010) and discussed (Hazen et al., 2010; Vynne et al., 2011). The antibacterial activity of the 12 bioactive strains remaining was quantified using a 96-well micro-titration method (Wiegand et al.,

2008). Insofar as the chemical nature of the active compounds was unknown, MICs were expressed as a function of maximal dilution factor of the supernatant that exerted a total EX527 inhibition of pathogen growth. MICs were also expressed in protein concentration (Table 3). All the hCg strains and hMe-187 and -253 supernatants were able to inhibit 100% of bacterial growth of at least one pathogen when diluted at least 64-fold. Moreover, eight haemolymph-associated PD184352 (CI-1040) isolates inhibited at least five different species among the seven Vibrio species included in the panel and one or more other bacteria, suggesting a real potential for antibacterial treatment in aquaculture farming, since Vibrio species are pathogenic for fish (Toranzo et al.,

2005), molluscs (Verschuere et al., 2000) and crustaceans (Wang, 2011). The active haemolymph-associated strains, hCg-23, -48, -51, -108, -109, hPm-26, hMe-95, -223, -253 and -273, were identified by 16S rRNA gene amplification as members of the Gammaproteobacteria class (Fig. 1) belonging to either the Alteromonadales (89%) or the Vibrionales orders (11%). Such affiliation was also observed in antimicrobial screening of marine bacteria and in previously described probiotics used in bivalve hatcheries (Zheng et al., 2005; Gram et al., 2010; Prado et al., 2010; Wilson et al., 2010; Flemer et al., 2012). Vibrio genus has been described to be a natural flora in bivalve and crustacean haemolymph (Faury et al., 2004; Gay et al., 2004; Gomez-Gil et al., 2010). The antibacterial as well as probiotic ability of this genus has been described (Riquelme et al., 1997, 2001; Mansson et al., 2011; Silva-Aciares et al., 2011). Nine strains, hCg-23, -48, -51, -108, -109, hMe-95, -223, -253 and -273, were affiliated with the Pseudoalteromonas genus.

The median age at transition to adult HIV services in the UK is 1

The median age at transition to adult HIV services in the UK is 17 years [3]; these pregnancies were reported both from paediatric settings and following transition to adult services, with the

median age at first pregnancy being 18 years. In three-quarters of the pregnancies women were reported to have detectable virus close to conception, with potential associated risk of transmission to partners; only half of the partners were reported by healthcare professionals to be aware of the woman’s status up to the time of delivery. While poor uptake of contraception and difficulties with partner disclosure are not limited to adolescence, professionals may need to reconsider their approach to educating this AZD0530 price cohort about contraception and partner disclosure, and consider recommending

effective long-acting reversible contraception in this population. While Liproxstatin-1 barrier contraception is required to reduce the risk of HIV transmission to sexual partners, use is often inconsistent and concentrating on promoting condom use may detract from offering other more effective methods of contraception. Adherence to therapy was reported to be suboptimal at some stage in about half the pregnancies described, with at least one woman requiring hospital admission for directly observed therapy. Problems with attendance and adherence are common during adolescence for many chronic childhood conditions and result in increased disease-related morbidity and mortality [3, 11]. Adolescents living with HIV have poorer adherence to cART compared with children or older adult populations, and poor TCL adherence has also been associated with depression, alcohol and substance abuse, and lack of wider disclosure of HIV status [11, 12]. cART is effective in preventing first-generation MTCT of HIV with overall MTCT rates < 1% with optimal care [13]. In this cohort a single infant was infected, comparable to other reported adolescent cohorts in the USA (one of 30) [9] and a predominantly horizontally infected UK cohort (one

of 66) [10]. Five young women delivered with detectable virus, increasing the risk of transmission to their babies. Multidisciplinary care with the aim of improving adherence to cART during adolescence and particularly during pregnancy should remain a priority; complex social circumstances with frequent social service involvement and high rates of mental health illness should be considered when planning adherence interventions. The rate of preterm deliveries (14%) in this cohort was almost twice the overall European rate in adolescents [14, 15] but similar to the overall rate reported for HIV-positive women in the UK and Ireland [4]. Data are currently sparse on the prevalence of congenital abnormalities in the offspring of perinatally infected adolescents.

, 2003; Galhardo et al, 2005) Therefore, the dnaE2-containing g

, 2003; Galhardo et al., 2005). Therefore, the dnaE2-containing gene cluster has also been named as a ‘mutagenesis cassette’ (Erill et al., 2006). The fact that the presence of the ‘mutagenesis cassette’ coincides with the lack of umuDC genes in the bacterial genome has suggested that this gene cassette

might functionally replace the absence of Pol V in these species by playing a role in TLS (Erill et al., 2006). The results presented in Alonso et al. (1999) showed that the emergence of multidrug-resistant mutants in P. aeruginosa increases under antibiotic challenge. Nonlethal concentrations of antibiotics have been suggested to enhance mutations conferring antibiotic find more resistance via the induction of specialized DNA polymerases (Couce & Blázquez, 2009). For example, Pol IV is induced by ceftazidime, a PBP3 inhibitor (Blázquez et

al., 2006). The role of specialized DNA polymerases in stationary-phase mutagenesis in Pseudomonas species under carbon starvation conditions has mostly been investigated using a P. putida model (e.g. Tegova et al., 2004; Tark et al., 2005; Koorits et al., 2007). The assay systems used in P. putida enable to isolate phenol-degrading Phe+ revertants due to the activation of a silent phenol monooxygenase gene pheA on a plasmid under carbon starvation conditions on minimal agar plates containing phenol as the only carbon source (Kasak et al., 1997; Tegova et al., 2004). Among the P. putida DNA polymerases, Pol IV is specifically involved in the generation of frameshift mutations under Erlotinib the carbon starvation conditions, but has no effects on the frequency of occurrence of base substitutions (Tegova et al., 2004). Differently from

the Pol IV-dependent stationary-phase mutations in E. coli, the occurrence of 1 bp deletions in starving P. putida cells does not depend on RecA functionality, nor does it require the stationary-phase sigma factor RpoS (Tegova et al., 2004; Tarassova et al., 2009). Notably, the Pol IV-dependent mutagenesis is remarkably elevated in P. putida populations starved for >1 week. This indicates that the level of expression of the mutagenic activity of Pol IV or certain Thiamet G type of DNA damage serving as a substrate for TLS by Pol IV might be increased during the long-term carbon starvation of P. putida. As already noted above, DnaE2 has been considered as an error-prone DNA polymerase (Boshoff et al., 2003; Galhardo et al., 2005). Unexpectedly, the frequency of accumulation of base substitution mutations was up to three times elevated in the DnaE2-deficient P. putida during the 10-day carbon starvation period studied (Koorits et al., 2007). The antimutator effect of DnaE2 also occurred using the chromosomal Rifr assay, which enables to detect base substitution mutations in the rpoB gene. UV-irradiated cells of the DnaE2-deficient P.

All travelers are advised of any follow-up immunizations that nee

All travelers are advised of any follow-up immunizations that need to be scheduled and are reminded if they should have any questions regarding any of the topics reviewed, they may call the clinic any time before or after their travel. There are limitations of this travel health Erlotinib ic50 clinic. Currently the travel health clinic is only open once a week despite the ambulatory clinic being open every day. The number of visits to the clinic was initially low, but

proper advertising has increased the number of patient appointments, as of October 2010 over 100 patients have been seen at the clinic. Current local regulations prevent the pharmacist from administering any immunizations other than the influenza vaccine. The benefits of having a multidisciplinary approach are many. The pharmacy students and patients may benefit the most with this unique team approach at the travel clinic. The students selleck chemicals llc have the opportunity to

apply what they have learned in a didactic class to a very specialized field of medicine that focuses on disease prevention and health promotion. They learn about emerging infectious diseases, their risks, and patterns of resistance. They learn how to access the most current travel-related information and work with a team to benefit the patient based solely on their individual needs. They truly learn the core values of any travel health specialist: individual risk assessment, educating

and communicating with the patient on disease prevention, and how to be safe during travel. At a time of globalization, this training may be invaluable to the patients they may serve in the future. The patients benefit by having an in-depth Buspirone HCl pretravel consultation by multiple healthcare providers each with their own area of expertise. It is our hope that the patients come away from their pretravel consultation with a better understanding of how to remain healthy during their trip and what to expect from the medications and immunizations they received. The authors state they have no conflicts of interest to declare. “
“2010 Ed , (ii) +398 pp , softcover, GBP 45.00 , ISBN 978-095657920-1 , London , National Travel Health Network and Center , 2010 . Following in the tradition of International Travel and Health1 and Health Information for International Travel,2Health Information for Overseas Travel is the latest addition to the exclusive portfolio of major guidelines in travel health. The completely revised 2010 edition of Health Information for Overseas Travel is a major update of what is known in the UK as the “Yellow Book.” It has a table of Contents, a Preface, six main sections, a comprehensive index, and an Acknowledgements and a Disclaimer on the inside back cover.

All travelers are advised of any follow-up immunizations that nee

All travelers are advised of any follow-up immunizations that need to be scheduled and are reminded if they should have any questions regarding any of the topics reviewed, they may call the clinic any time before or after their travel. There are limitations of this travel health check details clinic. Currently the travel health clinic is only open once a week despite the ambulatory clinic being open every day. The number of visits to the clinic was initially low, but

proper advertising has increased the number of patient appointments, as of October 2010 over 100 patients have been seen at the clinic. Current local regulations prevent the pharmacist from administering any immunizations other than the influenza vaccine. The benefits of having a multidisciplinary approach are many. The pharmacy students and patients may benefit the most with this unique team approach at the travel clinic. The students A-769662 price have the opportunity to

apply what they have learned in a didactic class to a very specialized field of medicine that focuses on disease prevention and health promotion. They learn about emerging infectious diseases, their risks, and patterns of resistance. They learn how to access the most current travel-related information and work with a team to benefit the patient based solely on their individual needs. They truly learn the core values of any travel health specialist: individual risk assessment, educating

and communicating with the patient on disease prevention, and how to be safe during travel. At a time of globalization, this training may be invaluable to the patients they may serve in the future. The patients benefit by having an in-depth Rutecarpine pretravel consultation by multiple healthcare providers each with their own area of expertise. It is our hope that the patients come away from their pretravel consultation with a better understanding of how to remain healthy during their trip and what to expect from the medications and immunizations they received. The authors state they have no conflicts of interest to declare. “
“2010 Ed , (ii) +398 pp , softcover, GBP 45.00 , ISBN 978-095657920-1 , London , National Travel Health Network and Center , 2010 . Following in the tradition of International Travel and Health1 and Health Information for International Travel,2Health Information for Overseas Travel is the latest addition to the exclusive portfolio of major guidelines in travel health. The completely revised 2010 edition of Health Information for Overseas Travel is a major update of what is known in the UK as the “Yellow Book.” It has a table of Contents, a Preface, six main sections, a comprehensive index, and an Acknowledgements and a Disclaimer on the inside back cover.

Lipodystrophy was evaluated according to this categorization in t

Lipodystrophy was evaluated according to this categorization in the face, arms, legs, buttocks, abdomen, neck and breasts. The sum of the values corresponding to each corporal zone indicated the degree of lipodystrophy: nonexistent (0), slight (1–6), moderate (7–12) and severe (13–18). In this study we included only moderate and severe cases in order to avoid an overlap between the LD+ and LD− subsets. The LD+ group comprised 26 patients with pure lipoatrophy and 106 patients with Z-IETD-FMK molecular weight the mixed type. No cases of pure lipohypertrophy were recorded.

With respect to severity, 109 had moderate and 23 had severe lipodystrophy. After an overnight fast, 20 mL of blood obtained from a peripheral vein was collected in Vacutainer™ (Becton Dickinson Immunocytometry Systems, San Jose, CA, USA) ethylenediaminetetraacetic acid (EDTA) tubes. Five millilitres of whole blood was used to determine the CD4 T-cell count. Five hundred microlitres was used for DNA isolation with a MagNa Pure LC Instrument (Roche Diagnostics, selleck kinase inhibitor Basel, Switzerland). Plasma and serum were obtained by centrifugation at 3500 g for 15 min at 4 °C and stored at −80 °C until use. HIV-1 infection and plasma HIV-1 viral load were assessed as described elsewhere [14]. The CD4 T-cell count was determined using a flow cytometer FAC Scan (Becton Dickinson Immunocytometry Systems). Data acquired were analysed using the multiset program

(Becton Dickinson Immunocytometry Systems). Plasma glucose, total cholesterol, HDL cholesterol and triglycerides were determined in an ADVIA 1200 (Siemens AG, Munich, Germany) auto-analyser using standard enzyme methods. Low-density lipoprotein (LDL) cholesterol was calculated using

the Friedewald formula [16]. Fasting plasma insulin was measured Etoposide using a specific immunoradiometric assay (Medgenix Diagnostics, Fleunes, Belgium) in which proinsulin did not cross-react. The intra- and inter-assay coefficients of variation (CVs) were 6% and 7%, respectively. The homeostasis model assessment of insulin resistance (HOMA-IR) as a marker for insulin resistance was calculated according to the formula [fasting glucose (in millimoles per litre) × fasting insulin (in microunits per millilitre)/22.5] [17]. Soluble tumour necrosis factor receptor 1 (sTNF-R1) and sTNF-R2 were assessed as previously described [18]. Adiponectin levels were measured using a standardized radioimmunoassay kit from Linco Research (Linco Research Inc., St. Charles, MO, USA). The kit has a sensitivity of 1 ng/mL. The intra- and inter-assay CVs were 8% and 12%, respectively. Plasma FABP-4 was measured using the Human Adipocyte FABP ELISA (BioVendor Laboratory Medicine, Palackeho, Czech Republic). The sensitivity was 0.1 ng/mL. The intra- and inter-assay CVs were 5.2% and 3.8%, respectively. The leptin concentration in plasma was determined with a Human Leptin ELISA kit (Assaypro, St Charles, MO, USA); the lowest detectable level was 0.15 pg/mL with an intra-assay CV of 4.

A similar route has been demonstrated for the selenate-respiring

A similar route has been demonstrated for the selenate-respiring bacterium T. selenatis (Lowe et al., 2010). In T. selenatis, electrons are mediated between membrane-bound quinol:cytochrome c oxidoreductase (bc1-complex) and periplasmic selenate reductase (Ser) by the 24-kDa di-heme cytochrome cytc-Ts4. In the photosynthetic bacterium R. sulfidophilum, electrons are transferred in the opposite direction on the oxidation of dimethyl sulfide to DMSO. Electrons

are shuttled from the periplasmic DMS dehydrogenase to the membrane-bound photosynthetic reaction center, mediated by the soluble cytochrome c2 (Creevey et al., 2008). In the present paper, we describe the purification and characterization of the cytochrome c discussed above. MS gives a molecular weight of about 9 kDa rather than selleck compound the 6 kDa found by SDS-PAGE. We denote this protein cytochrome c-Id1. Electron transfer to chlorate is thermodynamically feasible from its EX 527 concentration estimated redox potential, and we demonstrate its ability to serve as electron donor for purified chlorate reductase. Ideonella dechloratans was obtained from the Culture Collection of Göteborg University, Göteborg, Sweden. Cells were cultured anaerobically (8 L) and harvested by centrifugation at 8000 g for 15 min. Wet

cells (20 g) were resuspended in 90 mL of 0.3 M Tris-HCl, pH 8.1, containing 20% (w/v) sucrose and 1 mM EDTA. The suspension was placed at room temperature for 10 min, followed by centrifugation at 8000 g for 10 min. The pellet was resuspended in 90 mL ice-cold 0.5 mM MgCl, and was kept on ice for 10 min.

Soluble proteins were extracted by from centrifugation at 8000 g for 20 min and ammonium sulfate was added to the protein extract to 40% saturation. The solution was stirred on ice for 30 min, followed by centrifugation at 18 000 g for 10 min. Ammonium sulfate was added to the supernatant to 85% saturation and the solution was stirred on ice for 30 min. Precipitated proteins were pelleted by centrifugation at 18 000 g for 10 min, and resuspended in 10 mL sodium phosphate (50 mM, pH 7.0) containing ammonium sulfate (0.92 M). The solution was applied on to a Phenyl Sepharose 6 Fast Flow (low sub) column, 20 × 2.6 cm (GE Healthcare, Uppsala, Sweden) at a flow rate of 1 mL min−1. The column was washed with 60 mL sodium phosphate/ammonium sulfate (50 mM/0.92 M, pH 7.0) and was eluted using a gradient of 500 mL (0.92–0 M ammonium sulfate) at a flow rate of 2 mL min−1. The cytochrome c was eluted at approximately 0.37 M ammonium sulfate. Appropriate fractions were pooled and concentrated using an Amicon stirred cell 8050 with Ultrafiltration Membrane, MWCO 1000 Da (Millipore, Solna, Sweden). The concentrated sample was applied on to a Sephacryl S-200 Hiprep™ 16/60 column (GE Healthcare) and eluted using 0.1 M sodium phosphate, pH 7.0, with the flow rate of 0.1 mL min−1.