Am J Clin Nutr 2004,80(Suppl):1678S-1688S.PubMed 3. Heaney RP: Vitamin D and calcium interactions: functional outcomes. Am J Clin Nutr 2008,88(Suppl):541S-544S.PubMed 4. Adams JS, Hewison M: Update in vitamin D. J Clin Endocrinol Metab 2010, 95:471–478.PubMedCrossRef 5. Foo LH, Zhang

Q, Zhu K, Ma G, Hu X, Greenfield H, Fraser DR: Low vitamin D status has an adverse influence on bone mass, bone turnover, and muscle strength in chinese adolescent girls. J Nutr 2009, 139:1002–1007.PubMedCrossRef selleck chemical 6. Craney A, Weiler HA, O’Donnell S, Puil L: Summary of evidence-based review on vitamin D efficacy and safety in relation to bone health. Am J Clin Nutr 2008,88(Suppl):513S-519S. 7. Ruohola JP, Laakksi I, Ylikomi T, Haatja R, Mattila VM, Sahi T, Tuohimaa P, Pihlajamaki H: VX-661 supplier Association between serum 25(OH)D concentrations and bone stress fractures in Finnish young men. J Bone Miner Res 2006, 21:1483–1488.PubMedCrossRef 8. McClung JP, Karl JP: Vitamin D and stress fracture: the contribution of vitamin D receptor gene polymorphisms. Nut Rev 2010, 68:365–369.CrossRef 9. Wentz L, Pei-Yang L, Haymes Staurosporine research buy E, Ilich JZ: Females have greater

incidence of stress fractures than males in both military and athletic populations: A systematic review. Mil Med 2011, 176:420–430.PubMed 10. Evans RK, Antczak AJ, Lester M, Yanovich R, Israeli E, Moran DS: Effects of a 4-month recruit training program on markers of bone metabolism. Med Sci Sports Exerc 2008,1(Suppl):660S-670S. 11. Andersen

NE, Karl JP, Cable SJ, Williams KW, Rood JC, Young AJ, Lieberman HR, McClung JP: Vitamin D status in female military personnel during combat training. J Int Soc Sports Nutr 2010, 7:38.PubMedCrossRef 12. Lappe J, Cullen D, Haynatzki G, Recker R, Ahlf R, Thompson K: Calcium and vitamin D supplementation decreases incidence of stress fractures in female Navy recruits. J Bone Miner Res 2008, 5:741–749.CrossRef mafosfamide 13. Jones BH, Canham-Chervak M, Canada S, Mitchener TA, Moore S: Medical surveillance of injuries in the US military: descriptive epidemiology and recommendations for improvement. Am J Prev Med 2010, 38:42S-60S.CrossRef 14. Burgi AA, Gorham ED, Garland CF, Mohr SB, Garland FC, Zeng K, Thompson K, Lappe JM: High serum 25-hydroxyvitamin D is associated with a low incidence of stress fractures. J Bone Miner Res 2011, 26:2371–2377.PubMedCrossRef 15. Harris SS, Dawson-Hughes B: Seasonal changes in plasma 25-hydroxyvitamin D concentrations of young American black and white women. Am J Clin Nutr 1998, 67:1232–1236.PubMed 16. Pasiakos SM, Karl JP, Lutz LJ, Andersen NE, Margolis LM, Rood JC, Cable SJ, Williams KW, Young AJ, McClung JP: Cardiometabolic risk in US Army recruits and the effects of basic combat training. PLoS One 2012, 7:e31222.PubMedCrossRef 17.

20 μm in diameter. Like other free-living ciliates, G. trihymene has eFT-508 mouse a transcriptionally active macronucleus and a germline micronucleus. The infraciliature

and buccal apparatus are the same as in previous find more reports, however, we found the life cycle was much more complicated and included two reproductive modes new to scuticociliates, asymmetric division and reproductive cysts. Figure 1 G. trihymene morphotypes. A, C, E were from living cells; B, D, F- H were from protargol impregnated specimens. A, B. Lateral and ventral view of trophonts. C. A well-fed trophont. D. One probable asymmetric divider. Arrow marks the smaller macronucleus. The white square frame marks the micronucleus from a different plane of focus. The smaller macronucleus differs

from the micronucleus by having many nucleoli. E, F. Ventral view of tomites. G. One asymmetric divider with two displaced macronuclei. H. One long asymmetric divider, probably releasing one trophont (arrow). Scale bars: A-H: 25 μm. Processes of asymmetric division in young cultures Many slowly moving, well-fed trophonts (Figure 1C) appeared within 24 hours after inoculation with tomites in cultures of wheat grain medium. In all of the cultures, a trophont underwent a cell division, but cytokinesis was arrested prior to completion, creating a unit consisting of two cells, now called “”subcells”" because of their failure to separate. PF-6463922 purchase Typically,

each of the two connected subcells later underwent a second transverse Selleckchem Forskolin division, resulting in a chain of four subcells, each with a macronucleus, an oral apparatus, and a contractile vacuole (Figures 1H; 2A). We define these chains of subcells as asymmetric dividers. Asymmetric dividers vary in sizes from 30 × 15 μm to 180 × 30 μm in vivo, have diverse shapes consisting of chains of 2-4 subcells (Figures 1G, H; 2A, J, O) and give rise to two filial cells that could be morphologically differentiated from each other after each division. Similar asymmetric dividers were also repeatedly found in different cultures, though the sizes varied with media type. Up to 4 macronuclei were found in the cytoplasm of each asymmetric divider (Figure 1H). Most undisturbed asymmetric dividers attached to the bottom of Petri dishes, moved very slowly or stayed immobile and had two or more rounded contractile vacuoles, pulsating with different frequencies (arrows in Figure 2C). The number of asymmetric dividers in the cultures increased with time from appearance of the first asymmetric divider. Figure 2 Division processes of two G.